PDF(Pubmed)
Abstract：
Lysosomes are the degradation centers and signaling hubs in the cell. Lysosomes undergo adaptation to maintain cell homeostasis in response to a wide variety of cues. Dysfunction of lysosomes leads to aging and severe diseases including lysosomal storage diseases (LSDs), neurodegenerative disorders, and cancer. To understand the complexity of lysosome biology, many research approaches and tools have been developed to investigate lysosomal functions and regulatory mechanisms in diverse experimental systems. This review summarizes the current approaches and tools adopted for studying lysosomes, and aims to provide a methodological overview of lysosomal research and related fields.
摘要：
溶酶体是细胞中的降解中心和信号中枢。响应于多种线索,溶酶体经历适应以维持细胞稳态。溶酶体的功能障碍导致衰老和严重的疾病，包括溶酶体贮积病（LSD），神经退行性疾病，和癌症。为了理解溶酶体生物学的复杂性，已经开发了许多研究方法和工具来研究不同实验系统中的溶酶体功能和调节机制。这篇综述总结了目前用于研究溶酶体的方法和工具，并旨在提供溶酶体研究和相关领域的方法学概述。
