RESULTS: Treatment with endosulfan at the dose of 3.5 mg/Kg body weight caused a higher degree of degeneration in the reproductive organ of Swiss albino mice . Treatment by this pesticide generated degeneration in long duration of dosage for 3,5 and 7 weeks. Ovaries of endosulfan administered groups showed degenerated germinal epithelium, Graffian follicles and corpus luteum. In testis of endosulfan treated mice, microscopic examination showed that there is significant damage and reduction in the tissue of seminiferous tubules and primordial germ cells. High degree of degeneration caused the disarrangement and deformation of spermatogonia with the decrease in the number of Sertoli cells. Biochemical and hormonal properties was also affected by endosulfan treatment. There was significant 5 folds decrease in the testosterone value of endosulfan in 7 weeks treated mice in comparison to control (p < 0.0001) and similarly there was significant elevation in the estrogen levels found in 7th week endosulfan treated mice. It also influenced the level of free radicals as there was significant decrease (p < 0.0001) in the value in catalase levels in 7 weeks endosulfan treated male and female mice, while significant (p < 0.0001) increase in the values of lipid peroxidation levels as 8 folds and 10 folds in 7 weeks endosulfan treated male and female Swiss albino mice respectively. This study hence speculates that the endosulfan exposed population are at the risk of reproductive health hazards.
CONCLUSIONS: The present study thus concludes that, endosulfan after 7 weeks of exposure caused significant reproductive damage to both male and female Swiss albino mice groups. Moreover, the karyotyping study also correlated the genotoxic damage in the mice.
结果:以3.5mg/Kg体重的剂量使用硫丹治疗会导致瑞士白化病小鼠生殖器官的变性程度更高。这种农药的治疗在3,5和7周的长期剂量中产生变性。硫丹给药组的卵巢显示退化的生发上皮,格拉夫卵泡和黄体。在硫丹治疗小鼠的睾丸中,显微镜检查表明,生精管和原始生殖细胞的组织有明显的损伤和减少。随着支持细胞数量的减少,高度变性引起精原细胞的排列和变形。生化和激素特性也受到硫丹处理的影响。与对照组相比,硫丹治疗7周的小鼠的睾酮值显着下降了5倍(p<0.0001),同样,硫丹治疗7周的小鼠的雌激素水平显着升高。它还影响了自由基的水平,因为在硫丹治疗7周的雄性和雌性小鼠中,过氧化氢酶水平的值显著降低(p<0.0001),而在经硫丹治疗7周的雄性和雌性瑞士白化病小鼠中,脂质过氧化水平的值分别显着(p<0.0001)增加了8倍和10倍。因此,这项研究推测硫丹暴露人群面临生殖健康危害的风险。
结论:本研究因此得出结论,硫丹暴露7周后,对雄性和雌性瑞士白化小鼠组造成了严重的生殖损害。此外,核型分析研究还与小鼠的遗传毒性损伤相关。