PDF(Pubmed)
Abstract：
BACKGROUND: Identification of animals in need of medical treatment is important in porcine health management, where analytical samples applicable at farm level could be utilized. Several biomarkers are measurable in saliva, which is less stressful to collect than blood. Saliva sampling is easy to learn and repeatable, making it suitable for monitoring purposes. Previous research suggests that porcine health biomarkers are dependent on production stage and gender, and that combining biomarkers improves diagnostic sensitivity. However, proper monitoring of biomarkers during the complete production cycle has not been studied. We aimed to describe the dynamics of salivary and serum haptoglobin (Hp), adenosine deaminase (ADA), and immunoglobulin G (IgG) in four production stages (suckling, early growing, late growing, finishing), on commercial Finnish pig farms using a total of 117 piglets. The relationship between gender and biomarker dynamics was investigated, as well as the relationships between these biomarkers in saliva and serum.
RESULTS: The highest salivary concentrations of Hp, ADA and IgG were measured in suckling piglets. The differences between production stages were generally larger in saliva than for the corresponding serum biomarkers. All correlation coefficients between salivary biomarkers were positive in each production stage and the strength of the correlation varied from 0.245 to 0.762. No similar trend was observed regarding correlation coefficients either between serum biomarkers or between salivary and serum biomarkers. Gender was associated with some biomarker concentrations.
CONCLUSIONS: The biomarker dynamics supported previous findings that collection of analytical samples should be conducted in age-matched populations. Positive and even strong relationships between salivary biomarkers indicate the potential to use especially saliva for health monitoring. Our results also suggest the importance of considering gender effects when assessing some salivary or serum biomarkers.
RESULTS: The highest salivary concentrations of Hp, ADA and IgG were measured in suckling piglets. The differences between production stages were generally larger in saliva than for the corresponding serum biomarkers. All correlation coefficients between salivary biomarkers were positive in each production stage and the strength of the correlation varied from 0.245 to 0.762. No similar trend was observed regarding correlation coefficients either between serum biomarkers or between salivary and serum biomarkers. Gender was associated with some biomarker concentrations.
CONCLUSIONS: The biomarker dynamics supported previous findings that collection of analytical samples should be conducted in age-matched populations. Positive and even strong relationships between salivary biomarkers indicate the potential to use especially saliva for health monitoring. Our results also suggest the importance of considering gender effects when assessing some salivary or serum biomarkers.
摘要：
背景：识别需要医疗的动物在猪的健康管理中很重要，可以利用适用于农场一级的分析样品。唾液中有几种生物标志物是可测量的，比血液收集压力小。唾液采样易于学习和重复，使其适用于监测目的。先前的研究表明，猪健康生物标志物取决于生产阶段和性别，结合生物标志物可以提高诊断灵敏度。然而,在整个生产周期中对生物标志物的适当监测尚未被研究。我们旨在描述唾液和血清结合珠蛋白（Hp）的动力学，腺苷脱氨酶(ADA),和免疫球蛋白G（IgG）在四个生产阶段（哺乳，早期生长,晚期生长，整理),在芬兰的商业养猪场上，总共使用了117只仔猪。研究了性别与生物标志物动态之间的关系，以及唾液和血清中这些生物标志物之间的关系。
结果：Hp的最高唾液浓度，在哺乳仔猪中测量ADA和IgG。唾液中生产阶段之间的差异通常大于相应的血清生物标志物。在每个生产阶段，唾液生物标志物之间的所有相关系数均为阳性，相关性的强度从0.245到0.762不等。关于血清生物标志物之间或唾液和血清生物标志物之间的相关系数没有观察到类似的趋势。性别与一些生物标志物浓度相关。
结论：生物标志物动力学支持先前的发现，即分析样本的收集应在年龄匹配的人群中进行。唾液生物标志物之间的积极甚至强烈的关系表明特别是唾液用于健康监测的潜力。我们的结果还表明在评估一些唾液或血清生物标志物时考虑性别影响的重要性。
结果：Hp的最高唾液浓度，在哺乳仔猪中测量ADA和IgG。唾液中生产阶段之间的差异通常大于相应的血清生物标志物。在每个生产阶段，唾液生物标志物之间的所有相关系数均为阳性，相关性的强度从0.245到0.762不等。关于血清生物标志物之间或唾液和血清生物标志物之间的相关系数没有观察到类似的趋势。性别与一些生物标志物浓度相关。
结论：生物标志物动力学支持先前的发现，即分析样本的收集应在年龄匹配的人群中进行。唾液生物标志物之间的积极甚至强烈的关系表明特别是唾液用于健康监测的潜力。我们的结果还表明在评估一些唾液或血清生物标志物时考虑性别影响的重要性。
