PDF(Pubmed)
Abstract：
Marek\'s disease virus (MDV) is a significant tumorigenic virus that causes severe immunosuppression in chickens. Lentinan (LNT) is an immunomodulator containing β-glucans and is widely used in areas such as antiviral, anticancer, and immune regulation. To investigate the immunomodulatory effects of LNT on specific pathogen-free (SPF) chicks and its potential to inhibit MDV infection, we conducted an MDV challenge experiment and observed the immune-enhancing effect of LNT on SPF chicks. The results showed that LNT promoted the growth and development of SPF chicks and induced the upregulation of cytokines such as Mx protein, interferon-γ (INF-γ), tumor necrosis factor-α (TNF-α), and interleukin-2 (IL-2). The specific gravity of CD4+ T-lymphocytes and CD8+ T-lymphocytes and their ratios were also significantly upregulated. Prophylactic use of LNT inhibited MDV replication in lymphocytes, liver, and spleen. It also alleviated MDV-induced weight loss and hepatosplenomegaly in SPF chicks. The present study confirms that LNT can enhance the levels of innate and cellular immunity in SPF chicks and contributes to the inhibition of MDV replication in vivo and mitigation of immune organ damage in chicks due to MDV infection. This provides an adjunctive measure for better control of MDV infection.
摘要：
马立克氏病病毒（MDV）是一种重要的致瘤病毒，可引起鸡的严重免疫抑制。香菇多糖(LNT)是一种含有β-葡聚糖的免疫调节剂,在抗病毒、抗癌，和免疫调节。探讨LNT对无特异性病原体(SPF)雏鸡的免疫调节作用及其抑制MDV感染的潜力。我们进行了MDV攻击实验，观察了LNT对SPF雏鸡的免疫增强作用。结果表明,LNT促进SPF雏鸡的生长发育,诱导Mx蛋白等细胞因子的上调,干扰素-γ（INF-γ），肿瘤坏死因子-α(TNF-α),和白细胞介素-2（IL-2）。CD4T淋巴细胞和CD8T淋巴细胞的比重及其比率也显着上调。预防性使用LNT抑制淋巴细胞中MDV的复制，肝脏,还有脾脏.它还减轻了MDV诱导的SPF雏鸡的体重减轻和肝脾肿大。本研究证实，LNT可以提高SPF雏鸡的先天免疫和细胞免疫水平，有助于抑制MDV在体内的复制，减轻由于MDV感染引起的雏鸡免疫器官损伤。这为更好地控制MDV感染提供了辅助措施。
