Abstract：
Caries is a destructive condition caused by bacterial infection that affects the hard tissues of the teeth, significantly reducing the quality of life for individuals. Photothermal therapy (PTT) offers a noninvasive and painless treatment for caries, but the use of unsafe laser irradiance limits its application. To address this challenge, we prepared nanoparticles of silver ion-doped Prussian blue (AgPB), which was encased within cationic guar gum (CG) to form the antibacterial PTT hydrogel CG-AgPB with a photothermal conversion efficiency of 34.4%. When exposed to an 808 nm laser at a power density of 0.4 W/cm2, the hydrogel readily reached a temperature of over 50 °C in just 3 min, synchronized by the discharge of Ag+ ions from the interstitial sites of AgPB crystals, resulting in broad-spectrum and synergistic antibacterial activities (>99%) against individual oral pathogens (Streptococcus sanguinis, Streptococcus mutans, and Streptococcus sobrinus) and pathogen-induced biofilms. In vivo, CG-AgPB-mediated PTT demonstrated a capability to profoundly reduce the terminal number of cariogenic bacteria to below 1% in a rat model of caries. Given the outstanding biocompatibility, injectability, and flushability, this CG-AgPB hydrogel may hold promise as a next-generation oral hygiene adjunct for caries management in a clinical setting.
摘要：
龋齿是由细菌感染引起的破坏性疾病，会影响牙齿的硬组织，显著降低个人生活质量。光热疗法（PTT）为龋齿提供了一种无创无痛的治疗方法，但是使用不安全的激光辐照限制了其应用。为了应对这一挑战，我们制备了银离子掺杂普鲁士蓝（AgPB）的纳米颗粒，将其包裹在阳离子瓜尔胶(CG)内以形成具有34.4%的光热转化效率的抗菌PTT水凝胶CG-AgPB。当暴露于功率密度为0.4W/cm2的808nm激光时，水凝胶很容易在3分钟内达到超过50°C的温度。由AgPB晶体间隙位点的Ag+离子放电同步，对个别口腔病原体（血链球菌，变形链球菌,和sobrinus链球菌）和病原体诱导的生物膜。在体内,CG-AgPB介导的PTT显示出在龋齿大鼠模型中将致龋细菌的末端数量显著减少至低于1%的能力。鉴于出色的生物相容性，可注射性,和可冲洗性，这种CG-AgPB水凝胶有望成为下一代口腔卫生辅助药物,用于临床治疗龋齿.
