UNASSIGNED: Using the human B-cell line Ramos (RA.1), various biological effects were evaluated with and without parathyroid hormone (PTH) stimulation at varying concentrations. Flow cytometry was employed to evaluate the phenotype of B lymphocytes based on IgD and CD38 expression, apoptosis induction via Annexin V and proliferation using CFSE. IgM production was quantified through ELISA, and Western blot analysis was performed to assess syk protein phosphorylation as an indicator of cell activation.
UNASSIGNED: Ramos cells (RA.1) evidenced a statistically significant change in the phenotype under human PTH stimulation, demonstrating an increased proportion of germinal centre cells (Bm3-Bm4) when stimulated with high concentrations of PTH.
UNASSIGNED: The in vitro effects of PTH in B cells subsets align with previous findings of an altered phenotype in B lymphocytes expressing PTH1R among autoimmune disease patients, suggesting a potential role of this hormone in the pathophysiology of autoimmune diseases. However, further studies are necessary to elucidate the mechanisms by which PTH generates observed effects in B lymphocytes and to determine if PTH plays a role in autoimmunity.
■使用人B细胞系Ramos(RA.1),在有和没有不同浓度的甲状旁腺激素(PTH)刺激的情况下,评估了各种生物学效应。采用流式细胞术评估基于IgD和CD38表达的B淋巴细胞表型,通过膜联蛋白V诱导凋亡和使用CFSE的增殖。IgM产生通过ELISA定量,进行蛋白质印迹分析以评估作为细胞活化指标的syk蛋白磷酸化。
■Ramos细胞(RA.1)在人PTH刺激下证明了表型的统计学显着变化,证明当用高浓度PTH刺激时,生发中心细胞(Bm3-Bm4)的比例增加。
■PTH在B细胞亚群中的体外作用与先前在自身免疫性疾病患者中表达PTH1R的B淋巴细胞表型改变的发现一致,提示这种激素在自身免疫性疾病的病理生理学中的潜在作用。然而,需要进一步的研究来阐明PTH在B淋巴细胞中产生观察到的作用的机制,并确定PTH是否在自身免疫中起作用.