PDF(Pubmed)
Abstract：
UNASSIGNED: The etiopathogenesis of autoimmune diseases is multifactorial, including hormonal factors. Remission of autoimmunity has been observed following treatment for concomitant hyperparathyroidism. Additionally, patients with autoimmune diseases have shown increased expression of parathyroid hormone receptor (PTH1R) and altered distribution of B cells subsets. Hence, this study aims to evaluate potential mechanisms and in vitro effects of PTH stimulation on B lymphocytes.
UNASSIGNED: Using the human B-cell line Ramos (RA.1), various biological effects were evaluated with and without parathyroid hormone (PTH) stimulation at varying concentrations. Flow cytometry was employed to evaluate the phenotype of B lymphocytes based on IgD and CD38 expression, apoptosis induction via Annexin V and proliferation using CFSE. IgM production was quantified through ELISA, and Western blot analysis was performed to assess syk protein phosphorylation as an indicator of cell activation.
UNASSIGNED: Ramos cells (RA.1) evidenced a statistically significant change in the phenotype under human PTH stimulation, demonstrating an increased proportion of germinal centre cells (Bm3-Bm4) when stimulated with high concentrations of PTH.
UNASSIGNED: The in vitro effects of PTH in B cells subsets align with previous findings of an altered phenotype in B lymphocytes expressing PTH1R among autoimmune disease patients, suggesting a potential role of this hormone in the pathophysiology of autoimmune diseases. However, further studies are necessary to elucidate the mechanisms by which PTH generates observed effects in B lymphocytes and to determine if PTH plays a role in autoimmunity.
UNASSIGNED: Using the human B-cell line Ramos (RA.1), various biological effects were evaluated with and without parathyroid hormone (PTH) stimulation at varying concentrations. Flow cytometry was employed to evaluate the phenotype of B lymphocytes based on IgD and CD38 expression, apoptosis induction via Annexin V and proliferation using CFSE. IgM production was quantified through ELISA, and Western blot analysis was performed to assess syk protein phosphorylation as an indicator of cell activation.
UNASSIGNED: Ramos cells (RA.1) evidenced a statistically significant change in the phenotype under human PTH stimulation, demonstrating an increased proportion of germinal centre cells (Bm3-Bm4) when stimulated with high concentrations of PTH.
UNASSIGNED: The in vitro effects of PTH in B cells subsets align with previous findings of an altered phenotype in B lymphocytes expressing PTH1R among autoimmune disease patients, suggesting a potential role of this hormone in the pathophysiology of autoimmune diseases. However, further studies are necessary to elucidate the mechanisms by which PTH generates observed effects in B lymphocytes and to determine if PTH plays a role in autoimmunity.
摘要：
■自身免疫性疾病的病因是多因素的，包括荷尔蒙因素。治疗伴随的甲状旁腺功能亢进后观察到自身免疫的缓解。此外,自身免疫性疾病患者显示甲状旁腺激素受体（PTH1R）表达增加，B细胞亚群分布改变。因此，这项研究旨在评估PTH刺激对B淋巴细胞的潜在机制和体外作用。
■使用人B细胞系Ramos（RA.1），在有和没有不同浓度的甲状旁腺激素（PTH）刺激的情况下，评估了各种生物学效应。采用流式细胞术评估基于IgD和CD38表达的B淋巴细胞表型,通过膜联蛋白V诱导凋亡和使用CFSE的增殖。IgM产生通过ELISA定量，进行蛋白质印迹分析以评估作为细胞活化指标的syk蛋白磷酸化。
■Ramos细胞（RA.1）在人PTH刺激下证明了表型的统计学显着变化，证明当用高浓度PTH刺激时，生发中心细胞（Bm3-Bm4）的比例增加。
■PTH在B细胞亚群中的体外作用与先前在自身免疫性疾病患者中表达PTH1R的B淋巴细胞表型改变的发现一致，提示这种激素在自身免疫性疾病的病理生理学中的潜在作用。然而,需要进一步的研究来阐明PTH在B淋巴细胞中产生观察到的作用的机制,并确定PTH是否在自身免疫中起作用.
■使用人B细胞系Ramos（RA.1），在有和没有不同浓度的甲状旁腺激素（PTH）刺激的情况下，评估了各种生物学效应。采用流式细胞术评估基于IgD和CD38表达的B淋巴细胞表型,通过膜联蛋白V诱导凋亡和使用CFSE的增殖。IgM产生通过ELISA定量，进行蛋白质印迹分析以评估作为细胞活化指标的syk蛋白磷酸化。
■Ramos细胞（RA.1）在人PTH刺激下证明了表型的统计学显着变化，证明当用高浓度PTH刺激时，生发中心细胞（Bm3-Bm4）的比例增加。
■PTH在B细胞亚群中的体外作用与先前在自身免疫性疾病患者中表达PTH1R的B淋巴细胞表型改变的发现一致，提示这种激素在自身免疫性疾病的病理生理学中的潜在作用。然而,需要进一步的研究来阐明PTH在B淋巴细胞中产生观察到的作用的机制,并确定PTH是否在自身免疫中起作用.
