PDF(Pubmed)
Abstract：
Rhipicephalus sanguineus, a repulsive obligate blood feeder, is a three-host tick inflicting tremendous damage. Blood-sucking initiates tick-pathogen-host interactions along with alterations in the expression levels of numerous bioactive ingredients. Key molecules regulating blood meals were identified using the transcriptomic approach. A total number of 744 transcripts showed statistically significantly differential expression including 309 significantly upregulated transcripts and 435 significantly downregulated transcripts in semiengorged female ticks compared to unfed ticks, all collected in 2021. The top 10 differentially upregulated transcripts with explicit functional annotations included turripeptide OL55-like protein, valine tRNA ligase-like protein and ice-structuring glycoprotein-like protein. The top 10 differentially down-regulated transcripts were uncharacterized proteins. Gene Ontology (GO) enrichment analysis revealed four associated terms in the cellular component category and 16 in the molecular function category among the top 20 terms. Differentially expressed genes (DEGs) were enriched in GO terms ID 0000323 (lytic vacuole) and ID 0005773 (vacuole). The top 20 enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways included metabolism, cellular processes, organismal systems and human diseases. The DEGs were enriched in the KEGG term ID: ko-04142 (lysosome pathway) associated with intracellular digestion in the tick midgut epithelium. Molecular markers annotated via comparative transcriptomic profiling were expected to be candidate markers for the purpose of tick control.
摘要：
血根虫,排斥的强制性血液喂食器，是一个三宿主的蜱虫造成巨大的伤害。吸血会引发蜱-病原体-宿主相互作用，并改变许多生物活性成分的表达水平。使用转录组学方法鉴定了调节血餐的关键分子。与未喂食的蜱相比，在半饱的雌性蜱中，总共744个转录本显示出统计学上显着的差异表达，包括309个显着上调的转录本和435个显着下调的转录本。全部在2021年收集。具有明确功能注释的前10个差异上调转录本包括TurripeptipOL55-like蛋白，缬氨酸tRNA连接酶样蛋白和冰结构糖蛋白样蛋白。前10个差异下调的转录物是未表征的蛋白质。基因本体论（GO）富集分析显示，在前20个术语中，细胞成分类别中有四个相关术语，分子功能类别中有16个相关术语。在GO项ID0000323(裂解液泡)和ID0005773(液泡)中富集差异表达基因(DEGs)。前20个丰富的京都基因和基因组百科全书（KEGG）途径包括代谢，细胞过程，有机系统和人类疾病。DEGs富含KEGG术语ID：ko-04142（溶酶体途径），与tick中肠上皮中的细胞内消化有关。通过比较转录组谱分析注释的分子标记被预期为用于蜱控制目的的候选标记。
