Abstract：
OBJECTIVE: To screen differentially expressed proteins (DEPs) in the saliva of Early childhood caries (ECC) with different degrees of severity.
METHODS: The proteomic profiles of salivary of children with ECC of varying severity by data independent acquisition data independent acquisition (DIA) technique. A total of 12 preschool children aged 3-5 years were included in this study.
RESULTS: In this study, a total of 15,083 peptides and 1944 proteins were quantified; The results of DEPs screening showed that 34 DEPs were identified between the group H and the group LC, including 18 up-regulated proteins and 16 down-regulated proteins; 34 DEPs were screened between the group H and the group HC, including 17 up-regulated proteins and 17 down-regulated proteins; 42 DEPs were screened between the group LC and the group HC, including 18 up-regulated proteins and 24 down-regulated proteins. Among these DEPs, we screened several key proteins that may play a role in ECC, such as MK, histone H4, TGFβ3, ZG16B, MUC20, and SMR-3B.
CONCLUSIONS: Salivary proteins, as important host factors of caries, are differentially expressed between the saliva of ECC children and healthy children. Specific DEPs are expected to become potential biomarkers for the diagnosis of ECC.
METHODS: The proteomic profiles of salivary of children with ECC of varying severity by data independent acquisition data independent acquisition (DIA) technique. A total of 12 preschool children aged 3-5 years were included in this study.
RESULTS: In this study, a total of 15,083 peptides and 1944 proteins were quantified; The results of DEPs screening showed that 34 DEPs were identified between the group H and the group LC, including 18 up-regulated proteins and 16 down-regulated proteins; 34 DEPs were screened between the group H and the group HC, including 17 up-regulated proteins and 17 down-regulated proteins; 42 DEPs were screened between the group LC and the group HC, including 18 up-regulated proteins and 24 down-regulated proteins. Among these DEPs, we screened several key proteins that may play a role in ECC, such as MK, histone H4, TGFβ3, ZG16B, MUC20, and SMR-3B.
CONCLUSIONS: Salivary proteins, as important host factors of caries, are differentially expressed between the saliva of ECC children and healthy children. Specific DEPs are expected to become potential biomarkers for the diagnosis of ECC.
摘要：
目的：筛选不同严重程度的儿童早期龋齿（ECC）唾液中差异表达的蛋白质（DEPs）。
方法：通过独立于数据的采集独立于数据的采集（DIA）技术，对不同严重程度的ECC儿童的唾液进行蛋白质组学分析。本研究共纳入12名3-5岁学龄前儿童。
结果：在这项研究中，共量化了15,083个肽和1944个蛋白质；DEP筛选结果显示，在H组和LC组之间鉴定了34个DEP，包括18个上调蛋白和16个下调蛋白;在H组和HC组之间筛选34个DEP,包括17个上调蛋白和17个下调蛋白;在LC组和HC组之间筛选42个DEP,包括18个上调蛋白和24个下调蛋白。在这些DEP中,我们筛选了几种可能在ECC中起作用的关键蛋白质，比如MK,组蛋白H4，TGFβ3，ZG16B，MUC20和SMR-3B。
结论：唾液蛋白，作为龋齿的重要宿主因素，在ECC儿童和健康儿童的唾液之间差异表达。特异性DEP有望成为ECC诊断的潜在生物标志物。
方法：通过独立于数据的采集独立于数据的采集（DIA）技术，对不同严重程度的ECC儿童的唾液进行蛋白质组学分析。本研究共纳入12名3-5岁学龄前儿童。
结果：在这项研究中，共量化了15,083个肽和1944个蛋白质；DEP筛选结果显示，在H组和LC组之间鉴定了34个DEP，包括18个上调蛋白和16个下调蛋白;在H组和HC组之间筛选34个DEP,包括17个上调蛋白和17个下调蛋白;在LC组和HC组之间筛选42个DEP,包括18个上调蛋白和24个下调蛋白。在这些DEP中,我们筛选了几种可能在ECC中起作用的关键蛋白质，比如MK,组蛋白H4，TGFβ3，ZG16B，MUC20和SMR-3B。
结论：唾液蛋白，作为龋齿的重要宿主因素，在ECC儿童和健康儿童的唾液之间差异表达。特异性DEP有望成为ECC诊断的潜在生物标志物。
