PDF(Pubmed)
Abstract：
Food allergy is widely recognized as a significant health issue, having escalated into a global epidemic, subsequently giving rise to the development of numerous additional complications. Currently, the sole efficient method to curb the progression of allergy is through the implementation of an elimination diet. The increasing number of newly identified allergens makes it harder to completely remove or avoid them effectively. The immunoreactivity of proteins of bacterial origin remains an unexplored topic. Despite the substantial consumption of microbial proteins in our diets, the immunologic mechanisms they might induce require thorough validation. This stands as the primary objective of this study. The primary objective of this study was to evaluate the effects of bacterial proteins on the intestinal barrier and immune system parameters during hypersensitivity induction in both developing and mature organisms. The secondary objective was to evaluate the role of lipids in the immunoreactivity programming of these bacterial proteins. Notably, in this complex, comprehensively designed in vitro, in vivo, and ex vivo trial, the immunoreactivity of various bacterial proteins will be examined. In summary, the proposed study intends to address the knowledge gaps regarding the effects of Lactobacillus microbial proteins on inflammation, apoptosis, autophagy, and intestinal barrier integrity in a single study.
摘要：
食物过敏被广泛认为是一个重要的健康问题，已经升级为全球流行病，随后导致许多其他并发症的发展。目前,抑制过敏进展的唯一有效方法是实施消除饮食。新鉴定的过敏原数量的增加使得难以完全去除或有效地避免它们。细菌来源的蛋白质的免疫反应性仍然是未探索的话题。尽管我们的饮食中大量消耗微生物蛋白，它们可能诱导的免疫机制需要彻底验证。这是本研究的主要目的。这项研究的主要目的是评估细菌蛋白在发育中和成熟生物超敏反应诱导过程中对肠道屏障和免疫系统参数的影响。第二个目的是评估脂质在这些细菌蛋白质的免疫反应性编程中的作用。值得注意的是,在这个复杂的，在体外全面设计，在体内，和离体试验，将检查各种细菌蛋白质的免疫反应性。总之,拟议的研究旨在解决有关乳酸菌微生物蛋白对炎症的影响的知识空白，凋亡，自噬,和肠道屏障完整性在一项研究中。
