PDF(Pubmed)
Abstract：
Fat content is an important trait in pig production. Adipose tissue and muscle are important sites for fat deposition and affect production efficiency and quality. To regulate the fat content in these tissues, we need to understand the mechanisms behind fat deposition. Laiwu pigs, a Chinese indigenous breed, have significantly higher fat content in both adipose tissue and muscle than commercial breeds such as Duroc. In this study, we analyzed the transcriptomes in adipose tissue and muscle of 21-d-old Laiwu and Duroc piglets. Results showed that there were 828 and 671 differentially expressed genes (DEG) in subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT), respectively. Functional enrichment analysis showed that these DEG were enriched in metabolic pathways, especially carbohydrate and lipid metabolism. Additionally, in the longissimus muscle (LM) and psoas muscle (PM), 312 and 335 DEG were identified, demonstrating enrichment in the cell cycle and metabolic pathways. The protein-protein interaction (PPI) networks of these DEG were analyzed and potential hub genes were identified, such as FBP1 and SCD in adipose tissues and RRM2 and GADL1 in muscles. Meanwhile, results showed that there were common DEG between adipose tissue and muscle, such as LDHB, THRSP, and DGAT2. These findings showed that there are significant differences in the transcriptomes of the adipose tissue and muscle between Laiwu and Duroc piglets (P < 0.05), especially in metabolic patterns. This insight serves to advance our comprehensive understanding of metabolic regulation in these tissues and provide targets for fat content regulation.
摘要：
脂肪含量是养猪生产中的重要性状。脂肪组织和肌肉是脂肪沉积的重要部位，影响生产效率和质量。为了调节这些组织中的脂肪含量，我们需要了解脂肪沉积背后的机制。莱芜猪,一种中国本土品种，在脂肪组织和肌肉中的脂肪含量明显高于商业品种如Duroc。在这项研究中,我们分析了21日龄莱芜和杜洛克仔猪脂肪组织和肌肉中的转录组。结果显示,在皮下脂肪组织(SAT)和内脏脂肪组织(VAT)中,有828个和671个差异表达基因(DEG),分别。功能富集分析表明,这些DEG富集在代谢途径中,尤其是碳水化合物和脂类代谢.此外,在最长肌（LM）和腰大肌（PM）中，鉴定了312和335°，展示细胞周期和代谢途径的富集。分析了这些DEG的蛋白质-蛋白质相互作用（PPI）网络，并鉴定了潜在的hub基因，例如脂肪组织中的FBP1和SCD以及肌肉中的RRM2和GADL1。同时,结果表明，脂肪组织和肌肉之间存在共同的DEG，例如LDHB，THRSP,DGAT2结果表明，莱芜仔猪和杜洛克仔猪脂肪组织和肌肉转录组存在显著差异（P<0.05）。尤其是在代谢模式中。这种见解有助于促进我们对这些组织中代谢调节的全面理解，并为脂肪含量调节提供目标。
