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Abstract：
In Taiwan, the use of radiocontrast medium for clinical image diagnosis recently surpassed one million times and the overall prevalence of radiocontrast hypersensitivity was ~7%. A microRNA (miRNA/miRs) is a small non-coding RNA molecule that mostly plays a suppressor role in cells. However, the roles of miRNA expression in radiocontrast-induced mast cells activation remains to be elucidated. The aim of the present study was to investigate the role of miRNA on radiocontrast-induced mast cell activation. Computed tomography radiocontrast, ultravist and mouse mast cell line, P815, were used in the present study. Cell viability was detected by CCK-8 experiment. Levels of histamine and β-hexosaminidase were measured by ELISA. miRNA expression was detected by miRNA sequencing and reverse transcription-quantitative PCR. The results showed that ultravist could increase histamine release and reduce intracellular β-hexosaminidase levels of mast cells. A total of 102 miRNAs could be significantly upregulated by ultravist stimulation. Selected candidate miRNAs for the validation included miR-19a-3p and miR-362-3p which were also increased expression following stimulation with ultravist. In conclusion, ultravist could induce mast cell activation through upregulation of miR-19a-3p and miR-362-3p. Thus, miR-19a-3p and miR-362-3p could be promising candidates for development as novel targets for preventing radiocontrast-induced allergy in the future.
摘要：
在台湾,最近，用于临床影像诊断的放射性造影剂的使用超过一百万次，放射性造影剂超敏反应的总患病率约为7％。microRNA(miRNA/miRs)是一种小的非编码RNA分子,其主要在细胞中起抑制作用。然而,miRNA表达在造影剂诱导的肥大细胞活化中的作用尚待阐明.本研究的目的是研究miRNA在放射性对比剂诱导的肥大细胞活化中的作用。计算机断层扫描放射造影，ultravist和小鼠肥大细胞系，P815用于本研究。通过CCK-8实验检测细胞活力。通过ELISA测量组胺和β-氨基己糖苷酶的水平。通过miRNA测序和逆转录-定量PCR检测miRNA的表达。结果表明，超速肌能增加肥大细胞的组胺释放，降低细胞内β-氨基己糖苷酶水平。通过超强刺激可以显著上调总共102个miRNA。所选择的用于验证的候选miRNA包括miR-19a-3p和miR-362-3p,其在用ultravist刺激后表达也增加。总之,Ultravist可通过上调miR-19a-3p和miR-362-3p诱导肥大细胞活化。因此，miR-19a-3p和miR-362-3p可能是有希望开发的候选物，作为未来预防放射性对比剂诱导的过敏的新靶标。
