PDF(Pubmed)
Abstract：
Introduction Effective root canal cleaning and sealing are essential for a successful endodontic procedure. For the purpose of disinfecting root canals, both herbal and non-herbal medications are recommended. This study aimed to analyze the antimicrobial and cytotoxic properties of biosynthesized silver nanoparticles (AgNPs) synthesized from Azadirachta indica/neem and chemically synthesized AgNPs from trisodium citrate (TSC) against oral pathogens to be further used as an irrigant in endodontic treatment. Materials and methods To synthesize A. indica AgNPs, powdered fresh A. indica leaves were weighed, added to double distilled water, heated for 30 minutes, and then combined with silver nitrate solution. TSC was also used to create TSC AgNPs. X-ray diffraction (XRD), scanning electron microscopy (SEM), ocular observation, and the ultraviolet-visible light (UV-vis) spectrum were used to characterize the AgNPs. Studies were conducted on the extract\'s characteristics, including its cytotoxicity and antibacterial activity. Results The hue shift and peak on the UV-vis spectrophotometer were signs that AgNPs were forming. The XRD pattern showed that the sample included crystalline AgNPs, mostly spherical ones. By using SEM, the presence of AgNPs was also verified. AgNPs that were synthesized showed antimicrobial efficacy against Enterococcus faecalis. Compared to chemically synthesized AgNPs, A. indica AgNPs showed lower minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values, a bigger zone of inhibition (ZOI), and less cytotoxic action. Conclusion This study demonstrates the minimal cytotoxicity and antibacterial activity of A. indica AgNPs against E. faecalis. This suggests that they might also be employed as root canal cleaners. Before experimenting with animals or cell lines in clinical trials for endodontic treatment, further research should be done.
摘要：
简介有效的根管清洁和密封对于成功的牙髓手术至关重要。为了消毒根管，建议使用草药和非草药药物。本研究旨在分析由印染/印本合成的生物合成银纳米颗粒（AgNPs）和由柠檬酸三钠（TSC）合成的化学合成的AgNPs对口腔病原体的抗菌和细胞毒性，以进一步用作根管治疗的冲洗剂。材料和方法合成A.indicaAgNPs，将粉状的新鲜A.indica叶称重，加入双蒸水，加热30分钟,然后与硝酸银溶液混合。TSC也用于产生TSCAgNP。X射线衍射(XRD)扫描电子显微镜（SEM），眼部观察,和紫外-可见光(UV-vis)光谱用于表征AgNPs。对提取物的特征进行了研究，包括其细胞毒性和抗菌活性。结果UV-vis分光光度计上的色调偏移和峰是AgNPs形成的迹象。XRD图谱表明样品中含有结晶的AgNPs,主要是球形的。通过使用SEM，AgNPs的存在也得到证实。合成的AgNP显示出抗粪肠球菌的抗微生物功效。与化学合成的AgNPs相比，A.indicaAgNPs显示较低的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）值，更大的抑制区(ZOI)，和较少的细胞毒性作用。结论本研究表明，in草AgNPs对粪肠球菌具有最小的细胞毒性和抗菌活性。这表明它们也可以用作根管清洁器。在用动物或细胞系进行牙髓治疗的临床试验之前，应该做进一步的研究。
