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Abstract：
UNASSIGNED: We aimed to evaluate and compare the diagnostic performance of RNA-mNGS and DNA-mNGS workflow in bacterial pneumonia, fungal pneumonia and tuberculosis.
UNASSIGNED: A total of 134 cases suspected pneumonia undergoing both DNA and RNA based mNGS of bronchoalveolar lavage fluid (BALF) and also traditional etiological examination were evaluated retrospectively.Sensitivity, specificity, PPV, NPV and accuracy rate of DNA and RNA based mNGS were estimated.
UNASSIGNED: In the diagnosis performance of bacterial pathogens in LRTIs,the specificity of RNA-mNGS was higher than that of DNA-mNGS(82.3 % vs. 61.9 %, P < 0.01). There was no significant difference of sensitivity between the two process(71.4 % vs. 85.7 %, P = 0.375).In the diagnosis performance of fungal pathogens in LRTIs,the specificity of RNA-mNGS was higher than that of DNA-mNGS (72.3 % vs. 27.3 %,p < 0.001). There was no significant difference of sensitivity between the two process(96.5 % vs. 98.8 %,p = 0.125).In the diagnosis performance of tuberculosis in LRTIs,the sensitivity of DNA-mNGS was higher than that of RNA-mNGS (91.7 % vs. 33.3 %,p = 0.016),the specificity was similar in the two process (100 %).
UNASSIGNED: RNA-mNGS may reduced the misdiagnosis rate of bacterial and fungal pathogens in LRTIs.Compared to RNA-mNGS, DNA-mNGS may could improve the diagnostic rate of tuberculosis.
UNASSIGNED: A total of 134 cases suspected pneumonia undergoing both DNA and RNA based mNGS of bronchoalveolar lavage fluid (BALF) and also traditional etiological examination were evaluated retrospectively.Sensitivity, specificity, PPV, NPV and accuracy rate of DNA and RNA based mNGS were estimated.
UNASSIGNED: In the diagnosis performance of bacterial pathogens in LRTIs,the specificity of RNA-mNGS was higher than that of DNA-mNGS(82.3 % vs. 61.9 %, P < 0.01). There was no significant difference of sensitivity between the two process(71.4 % vs. 85.7 %, P = 0.375).In the diagnosis performance of fungal pathogens in LRTIs,the specificity of RNA-mNGS was higher than that of DNA-mNGS (72.3 % vs. 27.3 %,p < 0.001). There was no significant difference of sensitivity between the two process(96.5 % vs. 98.8 %,p = 0.125).In the diagnosis performance of tuberculosis in LRTIs,the sensitivity of DNA-mNGS was higher than that of RNA-mNGS (91.7 % vs. 33.3 %,p = 0.016),the specificity was similar in the two process (100 %).
UNASSIGNED: RNA-mNGS may reduced the misdiagnosis rate of bacterial and fungal pathogens in LRTIs.Compared to RNA-mNGS, DNA-mNGS may could improve the diagnostic rate of tuberculosis.
摘要：
■我们旨在评估和比较RNA-mNGS和DNA-mNGS工作流程在细菌性肺炎中的诊断性能，真菌性肺炎和肺结核.
■对134例疑似肺炎患者进行了基于DNA和RNA的支气管肺泡灌洗液（BALF）mNGS和传统病因检查。灵敏度,特异性，PPV,估计了基于DNA和RNA的mNGS的NPV和准确率。
■在LRTIs中细菌病原体的诊断性能中，RNA-mNGS的特异性高于DNA-mNGS(82.3%vs.61.9%,P<0.01)。两种工艺的灵敏度无显著差异(71.4%vs.85.7%,P=0.375)。在LRTIs中真菌病原体的诊断性能中，RNA-mNGS的特异性高于DNA-mNGS(72.3%vs.27.3%,p<0.001)。两种工艺的灵敏度无显著差异(96.5%vs.98.8%,p=0.125）。在LRTIs结核病的诊断表现中，DNA-mNGS的敏感性高于RNA-mNGS(91.7%vs.33.3%,p=0.016),两种方法的特异性相似(100%).
■RNA-mNGS可降低LRTI中细菌和真菌病原体的误诊率。与RNA-mNGS相比，DNA-mNGS可以提高结核病的诊断率。
■对134例疑似肺炎患者进行了基于DNA和RNA的支气管肺泡灌洗液（BALF）mNGS和传统病因检查。灵敏度,特异性，PPV,估计了基于DNA和RNA的mNGS的NPV和准确率。
■在LRTIs中细菌病原体的诊断性能中，RNA-mNGS的特异性高于DNA-mNGS(82.3%vs.61.9%,P<0.01)。两种工艺的灵敏度无显著差异(71.4%vs.85.7%,P=0.375)。在LRTIs中真菌病原体的诊断性能中，RNA-mNGS的特异性高于DNA-mNGS(72.3%vs.27.3%,p<0.001)。两种工艺的灵敏度无显著差异(96.5%vs.98.8%,p=0.125）。在LRTIs结核病的诊断表现中，DNA-mNGS的敏感性高于RNA-mNGS(91.7%vs.33.3%,p=0.016),两种方法的特异性相似(100%).
■RNA-mNGS可降低LRTI中细菌和真菌病原体的误诊率。与RNA-mNGS相比，DNA-mNGS可以提高结核病的诊断率。
