PDF(Pubmed)
Abstract：
In the present study, in order to expand the knowledge on the distribution of parasites of the genus Eustrongylides in never investigated Italian lacustrine areas of Tuscany and Latium, fish specimens from four Tuscan lakes (Bilancino, Chiusi, Montedoglio and San Cipriano) and two Latium lakes (Bolsena and Bracciano) were collected and analyzed. The parasitological analysis, consisting of a visual inspection followed by a chloro-peptic digestion, was performed on 1650 specimens belonging to 17 species: European perch (Perca fluviatilis), Pumpkinseed (Lepomis gibbosus), Pike-perch (Sander lucioperca), Largemouth black bass (Micropterus salmoides), Pike (Esox lucius), Big-scale sand smelt (Atherina boyeri), Tench (Tinca), European Whitefish (Coregonus lavaretus), Channel catfish (Ictalurus punctatus), Chub (Squalius cephalus), Rudd (Scardinius erythrophthalmus), Common bleak (Alburnus alborella), South European roach (Sarmarutilus rubilio), South European nase (Protochondrostoma genei), Italian riffle dace (Telestes muticellus), Goldfish (Carassius auratus), and Freshwater bream (Abramis brama). Eustrongylides spp. larvae were only found in one specimen of European perch caught in Bracciano Lake and purchased from a local fishmonger (P = 3.3%; MI = 1; MA = 0.03). The only isolated Eustrongylides spp. larva was submitted to molecular analysis of the ITS gene region and identified as E. excisus. This finding adds Bracciano Lake to the list of the several Italian lakes in which nematodes belonging to this genus have been reported. Even if the observed prevalence is currently low, the presence of the parasite in this local freshwater fish supply chain requires FBOs to adopt risk management measures to prevent contaminated products from reaching final consumer. Moreover, future investigations will show if the parasite expands in these areas.
摘要：
在本研究中,为了扩大对从未调查过的意大利托斯卡纳和拉蒂姆湖相地区的Eustrongylides属寄生虫分布的了解，来自四个托斯卡纳湖泊的鱼类标本(Bilancino，Chiusi,收集并分析了Montedoglio和SanCipriano）和两个Latium湖（Bolsena和Bracciano）。寄生虫学分析,包括目视检查，然后进行氯消化，对属于17种物种的1650个标本进行了研究：欧洲鲈鱼（Percafluviatilis），南瓜籽（Lepomisgibbosus），派克鲈鱼(桑德·卢奇佩尔卡)，大嘴黑鲈鱼（小翅目），派克（埃索克斯·卢修斯），大型砂矿（Atherinaboyeri），Tench(Tinca),欧洲白鱼（Coregonuslavaretus），海峡鲶鱼(马氏Ictaluruspunctatus)，Chub(Squaluuscephalus),陆克文（红眼斯卡迪尼），普通的黯淡（Alburnusalborella），南欧蟑螂（Sarmarutilusrubilio），南欧nase（原软骨细胞基因），意大利riffledace（Telestesmuticellus），金鱼（Carassiusauratus），和淡水鲈鱼（Abramisbrama）。Eustrongylides属。仅在Bracciano湖捕获并从当地鱼贩那里购买的欧洲鲈鱼标本中发现了幼虫（P=3.3％；MI=1；MA=0.03）。唯一孤立的Eustrongylides属。对幼虫进行了ITS基因区域的分子分析，并鉴定为E。切除。这一发现将Bracciano湖添加到已报道属于该属的线虫的几个意大利湖泊中。即使观察到的患病率目前很低，当地淡水鱼供应链中寄生虫的存在要求FBO采取风险管理措施，以防止受污染的产品到达最终消费者。此外，未来的调查将显示寄生虫是否在这些地区扩张。
