关键词: Lichen Methylobacterium Thailand

来  源:   DOI:10.1016/j.dib.2024.110485   PDF(Pubmed)

Abstract:
A ubiquitous and pink-pigmented facultatively methylotrophic bacterium, designated LRY1-08 (=JCM 33120), was isolated from a lichen in Thailand. Strain LRY1-08 and Methylobacterium durans NBRC 112876T shared 99.92 % similarity based on the 16S rRNA gene sequence. The draft genome of LRY1-08 was 5.26 Mbp with 4,952 protein-coding sequences and an average G + C content of 70.0 mol%. Comparing strain LRY1-08 to M. durans NBRC 112876T, the ANIb, ANIm, AAI, and digital DNA-DNA hybridization values were 96.29 %, 97.10 %, 96.7 %, and 82.29 %, respectively. Based on the phenotypic characteristics and genome analysis, it was identified as M. durans. Its genomic sequence data revealed the PHB and CoQ10 biosynthesis genes. Therefore, the results offer suggestions for further investigation into possible applications of this bacterium in biotechnology. The draft genome was deposited at DDBJ/EMBL/GenBank (DNA Databank of Japan/European Molecular Biology Laboratory/Genbank) (JAYEEX000000000).
摘要:
一种普遍存在的粉红色色素兼性甲基营养细菌,指定LRY1-08(=JCM33120),是从泰国的地衣中分离出来的。基于16SrRNA基因序列,菌株LRY1-08和甲基芽孢杆菌NBRC112876T具有99.92%的相似性。LRY1-08的基因组草图为5.26Mbp,具有4,952个蛋白质编码序列,平均GC含量为70.0mol%。比较菌株LRY1-08与M.duransNBRC112876T,ANIb,ANIm,AAI,数字DNA-DNA杂交值为96.29%,97.10%,96.7%,和82.29%,分别。基于表型特征和基因组分析,被确认为M.Durans.其基因组序列数据揭示了PHB和CoQ10生物合成基因。因此,结果为进一步研究该细菌在生物技术中的可能应用提供了建议。基因组草案保存在DDBJ/EMBL/GenBank(日本DNA数据库/欧洲分子生物学实验室/Genbank)(JAYEEX000000000)。
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