PDF(Pubmed)
Abstract：
BACKGROUND: Verticillium wilt, caused by the fungus Verticillium dahliae, is a soil-borne vascular fungal disease, which has caused great losses to cotton yield and quality worldwide. The strain KRS010 was isolated from the seed of Verticillium wilt-resistant Gossypium hirsutum cultivar \"Zhongzhimian No. 2.\"
RESULTS: The strain KRS010 has a broad-spectrum antifungal activity to various pathogenic fungi as Verticillium dahliae, Botrytis cinerea, Fusarium spp., Colletotrichum spp., and Magnaporthe oryzae, of which the inhibition rate of V. dahliae mycelial growth was 73.97% and 84.39% respectively through confrontation test and volatile organic compounds (VOCs) treatments. The strain was identified as Bacillus altitudinis by phylogenetic analysis based on complete genome sequences, and the strain physio-biochemical characteristics were detected, including growth-promoting ability and active enzymes. Moreover, the control efficiency of KRS010 against Verticillium wilt of cotton was 93.59%. After treatment with KRS010 culture, the biomass of V. dahliae was reduced. The biomass of V. dahliae in the control group (Vd991 alone) was 30.76-folds higher than that in the treatment group (KRS010+Vd991). From a molecular biological aspect, KRS010 could trigger plant immunity by inducing systemic resistance (ISR) activated by salicylic acid (SA) and jasmonic acid (JA) signaling pathways. Its extracellular metabolites and VOCs inhibited the melanin biosynthesis of V. dahliae. In addition, KRS010 had been characterized as the ability to promote plant growth.
CONCLUSIONS: This study indicated that B. altitudinis KRS010 is a beneficial microbe with a potential for controlling Verticillium wilt of cotton, as well as promoting plant growth.
RESULTS: The strain KRS010 has a broad-spectrum antifungal activity to various pathogenic fungi as Verticillium dahliae, Botrytis cinerea, Fusarium spp., Colletotrichum spp., and Magnaporthe oryzae, of which the inhibition rate of V. dahliae mycelial growth was 73.97% and 84.39% respectively through confrontation test and volatile organic compounds (VOCs) treatments. The strain was identified as Bacillus altitudinis by phylogenetic analysis based on complete genome sequences, and the strain physio-biochemical characteristics were detected, including growth-promoting ability and active enzymes. Moreover, the control efficiency of KRS010 against Verticillium wilt of cotton was 93.59%. After treatment with KRS010 culture, the biomass of V. dahliae was reduced. The biomass of V. dahliae in the control group (Vd991 alone) was 30.76-folds higher than that in the treatment group (KRS010+Vd991). From a molecular biological aspect, KRS010 could trigger plant immunity by inducing systemic resistance (ISR) activated by salicylic acid (SA) and jasmonic acid (JA) signaling pathways. Its extracellular metabolites and VOCs inhibited the melanin biosynthesis of V. dahliae. In addition, KRS010 had been characterized as the ability to promote plant growth.
CONCLUSIONS: This study indicated that B. altitudinis KRS010 is a beneficial microbe with a potential for controlling Verticillium wilt of cotton, as well as promoting plant growth.
摘要：
背景：黄萎病，由真菌黄萎病菌引起的，是一种土壤传播的血管真菌病，给世界范围内的棉花产量和品质造成了巨大的损失。菌株KRS010是从抗黄萎病陆地棉品种的种子中分离得到的。2.\"
结果：菌株KRS010对各种病原真菌如黄萎病菌具有广谱抗真菌活性，灰葡萄孢菌,镰刀菌属。,炭疽病属。,和稻瘟病，其中通过对抗试验和挥发性有机化合物（VOCs）处理对大丽花菌丝生长的抑制率分别为73.97%和84.39%。通过基于完整基因组序列的系统发育分析，该菌株被鉴定为芽孢杆菌，并对菌株生理生化特性进行检测,包括生长促进能力和活性酶。此外，KRS010对棉花黄萎病的防治效果为93.59%。用KRS010培养物处理后，大丽花的生物量减少。对照组（单独的Vd991）的大丽花的生物量比处理组（KRS010Vd991）高30.76倍。从分子生物学的角度来看，KRS010可通过诱导水杨酸（SA）和茉莉酸（JA）信号通路激活的系统抗性（ISR）来触发植物免疫。其细胞外代谢产物和VOCs抑制大丽花黑色素的生物合成。此外,KRS010已被表征为促进植物生长的能力。
结论：这项研究表明，阿氏芽孢杆菌KRS010是一种有益的微生物，具有防治棉花黄萎病的潜力，促进植物生长。
结果：菌株KRS010对各种病原真菌如黄萎病菌具有广谱抗真菌活性，灰葡萄孢菌,镰刀菌属。,炭疽病属。,和稻瘟病，其中通过对抗试验和挥发性有机化合物（VOCs）处理对大丽花菌丝生长的抑制率分别为73.97%和84.39%。通过基于完整基因组序列的系统发育分析，该菌株被鉴定为芽孢杆菌，并对菌株生理生化特性进行检测,包括生长促进能力和活性酶。此外，KRS010对棉花黄萎病的防治效果为93.59%。用KRS010培养物处理后，大丽花的生物量减少。对照组（单独的Vd991）的大丽花的生物量比处理组（KRS010Vd991）高30.76倍。从分子生物学的角度来看，KRS010可通过诱导水杨酸（SA）和茉莉酸（JA）信号通路激活的系统抗性（ISR）来触发植物免疫。其细胞外代谢产物和VOCs抑制大丽花黑色素的生物合成。此外,KRS010已被表征为促进植物生长的能力。
结论：这项研究表明，阿氏芽孢杆菌KRS010是一种有益的微生物，具有防治棉花黄萎病的潜力，促进植物生长。
