Abstract：
The integration of recognition and therapeutic functions in multifunctional biosensors is of great importance in guaranteeing food security and reducing the occurrence of foodborne illness caused by foodborne pathogens. In this study, a biosensor utilizing a \"sense-and-treat\" approach was developed by integrating phage tailspike protein (TSP) with gold nanoparticles (AuNPs@TSP). The synthesized AuNPs@TSP showed strong binding affinity towards Salmonella typhimurium causing color changes and exhibited effective bactericidal activity when exposed to near-infrared (NIR) irradiation. This biosensor facilitated rapid colorimetric detection of S. typhimurium in 50 min, with a LOD (limit of detection) of 2.53 × 103 CFU/mL output on a smartphone APP after analyzing the red-green-blue (RGB) values from color rendering results. Furthermore, the biosensor displayed high selectivity, rapid response time, and broad applicability when tested with real samples. Moreover, the biosensor exhibited a remarkably efficient antibacterial efficacy of 100 % against S. typhimurium under 808 nm light irradiation for 6 min. This study provides a comprehensive investigation into the potential utilization of biosensors for rapid detection and eradication of foodborne pathogens in food industry.
摘要：
多功能生物传感器中识别和治疗功能的集成对于保障食品安全和减少食源性病原体引起的食源性疾病的发生具有重要意义。在这项研究中,通过将噬菌体尾穗蛋白（TSP）与金纳米颗粒（AuNPs@TSP）集成，开发了一种利用“感觉和治疗”方法的生物传感器。合成的AuNPs@TSP对鼠伤寒沙门氏菌表现出强烈的结合亲和力，引起颜色变化，并在暴露于近红外（NIR）辐射时表现出有效的杀菌活性。这种生物传感器有助于在50分钟内快速比色检测鼠伤寒沙门氏菌，在分析来自显色性结果的红-绿-蓝(RGB)值之后,智能手机APP上的LOD(检测限)为2.53×103CFU/mL输出。此外，生物传感器显示出高选择性，快速响应时间,和广泛的适用性，当测试与真实的样品。此外，该生物传感器在808nm光照6分钟下对鼠伤寒沙门氏菌表现出100%的显著有效抗菌效果。这项研究为生物传感器在食品工业中快速检测和根除食源性病原体的潜在利用提供了全面的调查。
