Abstract：
Cryptosporidium spp. is an important foodborne and waterborne pathogen in humans and animals, causing diarrhoea in humans and respiratory and gastrointestinal diseases in birds. However, reports of Cryptosporidium infection in bar-headed goose are limited. To determine the infection rate and species/genotypes of Cryptosporidium in bar-headed goose in China, a total of 358 fecal samples were collected from 3 regions. Nested PCR was used to amplify Cryptosporidium SSU rRNA regions from the fecal extracted-DNA samples. The total infection rate of Cryptosporidium in bar-headed in China was 3.9 % (14/358), with 4.2 % (5/120) in Aba (Ngawa) Tibetan and Qiang Autonomous Prefect, Sichuan province, 7.6 % (9/119) in Maqu county, Gansu province, and 0.0 % (0/119) in Caohai, Wei ning county, Guizhou province. The differences in prevalence rate by region were statistically significant. All positive samples were identified as Cryptosporidium goose genotype I (n = 14). This is the first systematic investigation of the epidemiological status and dominant species/genotypes of Cryptosporidium in bar-headed goose in China, thereby enhancing our understanding of the epidemiology of Cryptosporidium infection in wild migratory birds.
摘要：
隐孢子虫。是人类和动物中重要的食源性和水性病原体，引起人类腹泻和鸟类呼吸道和胃肠道疾病。然而,结头鹅隐孢子虫感染的报道有限.确定中国头鹅隐孢子虫的感染率和种类/基因型。共收集了3个地区的358份粪便样本。巢式PCR用于从粪便提取的DNA样品中扩增隐孢子虫SSUrRNA区域。我国柱头隐孢子虫总感染率为3.9%(14/358),阿坝（Ngawa）藏族和羌族自治州占4.2％（5/120），四川省,玛曲县7.6%(9/119)，甘肃省,和0.0%(0/119)在草海，魏宁县,贵州省。各地区的患病率差异有统计学意义。所有阳性样品均被鉴定为隐孢子虫鹅基因型I(n=14)。这是我国首次对斑头鹅隐孢子虫的流行病学现状和优势种/基因型进行系统的调查,从而增强我们对野生候鸟隐孢子虫感染流行病学的了解。
