Abstract：
Mesenchymal stromal cells (MSCs) have regenerative and immunomodulatory potential and may be used to treat injured tissues. Pregnancy has been associated with increased MSCs in the peripheral circulation in multiple species, but to date, there are no reports on this matter in horses. This study aimed to evaluate the effect of pregnancy on isolation efficiency and proliferation capacity of equine MSCs derived from the peripheral blood (PB) of mares. Venous blood samples were collected at the 11th month of gestation and 1 month after delivery from clinically healthy Arabian mares that presented normal pregnancies. Blood samples were processed for in vitro cellular culture and hormonal and metabolic profiles. MSCs were isolated and characterized by trilineage differentiation potential, immunophenotyping, analyzed by gene sequencing and proliferation assays. The isolation of peripheral blood mononuclear cells (PBMCs) of pregnant mares were associated with higher isolation efficiency and proliferative capacity of MSCs derived from peripheral blood (PB-MSCs) recovered pre-partum than those isolated post-partum. Although fetal gender, parity, 5α-reduced pregnanes, insulin, and cortisol were shown to affect cellular proliferation, individual factors and the small population studied must be considered. This study suggests that PB-MSCs from pregnant mares could be a valuable alternative source of MSCs for therapeutic purposes.
摘要：
间充质基质细胞（MSC）具有再生和免疫调节潜力，可用于治疗受损组织。在多个物种中，妊娠与外周循环中的MSCs增加有关，但迄今为止,马没有关于这件事的报道。本研究旨在评价妊娠对母马外周血(PB)来源的马MSCs分离效率和增殖能力的影响。在妊娠第11个月和分娩后1个月，从临床健康的阿拉伯母马中收集静脉血样。对血液样品进行处理以进行体外细胞培养以及激素和代谢谱。分离MSCs并通过三系分化潜能进行表征,免疫表型,通过基因测序和增殖试验进行分析。孕母外周血单核细胞（PBMC）的分离与产后恢复的外周血MSC（PB-MSC）的分离效率和增殖能力更高。虽然胎儿性别,奇偶校验，5α还原孕烷，胰岛素，皮质醇被证明会影响细胞增殖，必须考虑个体因素和研究的人口少。这项研究表明，来自怀孕母马的PB-MSC可能是用于治疗目的的MSC的有价值的替代来源。
