RESULTS: According to the Xpert Carba-R assay, 16 of the 55 isolates (29.1%) were categorised as Ambler Class A (11 of which matched CPO panel Class A identification); three isolates (5.5%) were identified as Class B and 27 isolates (49.1%) as Class D (in both cases consistent with CPO panel B and D classifications). A further eight isolates (14.5%) exhibited multiple carbapenemase enzymes and were designated as dual-carbapenemase producers, while one isolate (1.8%) was identified as a non-carbapenemase-producer. The CPO panel demonstrated positive and negative percent agreements of 100% and 85.7% for Ambler Class A, 100% and 100% for Class B, and 96.4% and 100% for Class D carbapenemase detection, respectively.
CONCLUSIONS: While the CPO panel\'s phenotypic performance was satisfactory in detecting Class B and D carbapenemases, additional confirmatory testing may be necessary for Class A carbapenemases as part of routine laboratory procedures.
结果:根据XpertCarba-R分析,55个分离株中的16个(29.1%)被归类为AmblerA类(其中11个与CPOA类鉴定相匹配);3个分离株(5.5%)被鉴定为B类,27个分离株(49.1%)被鉴定为D类(在两种情况下都与CPOB和D类分类一致)。另外8个分离株(14.5%)表现出多种碳青霉烯酶,并被指定为双碳青霉烯酶生产者,而一个分离物(1.8%)被鉴定为非碳青霉烯酶生产者。CPO小组显示,AmblerA类的正面和负面百分比协议分别为100%和85.7%,100%和100%对于B类,D类碳青霉烯酶检测为96.4%和100%,分别。
结论:虽然CPO小组的表型性能在检测B类和D类碳青霉烯酶方面令人满意,作为常规实验室程序的一部分,可能需要对A类碳青霉烯酶进行额外的验证性测试.