Abstract：
Euphorbia himalayensis Boiss. is an alpine member of the Euphorbiaceae family. Its dried roots have been used to treat digestive problems and chest congestion in traditional Tibetan and Mongolian medicine. Despite thousands of years of use in medicine, the bioactive compounds of the root remain unknown. Herein, we isolated a novel aqueous-soluble polysaccharide (EHP2) from the E. himalayensis root and determined its structural characteristics via high-performance gel permeation chromatography, Fourier-transform infrared spectroscopy, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectrometry. The homogeneous molecular weight of EHP2 was 23.6 kDa with narrow polydisperity (Mw/Mn = 1.4), and EHP2 mainly comprised of glucose (86.4%), galactose (11.9%) and mannose (1.7%). The major backbone of EHP2 was →4)-α-D-GalAp-(1 → 4)-α-D-Glcp-(1 → and the branch chain was α-D-Glcp-(1→. The antioxidant activity of the EHP2 was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and superoxide anion radical scavenging assays, and antioxidant enzyme activity (SOD, GSH and MDA) was determined in human umbilical vein endothelial cells (HUVECs). The EHP2 demonstrated lower potential scavenging effects on DPPH and superoxide free radical scavenger than ascorbic acid, and in HUVECs, it led to increased SOD and GSH activities and decreased MDA levels. This study is the first to describe an E. himalayensis polysaccharide compound with potential antioxidant activity.
摘要：
喜马拉雅一品红。是大齿科的高山成员。其干燥的根已被用于治疗传统的藏和蒙古医学中的消化问题和胸部充血。尽管在医学上使用了数千年，根的生物活性化合物仍然未知。在这里，我们从喜马拉雅根中分离出一种新型的水溶性多糖（EHP2），并通过高效凝胶渗透色谱法测定其结构特征，傅里叶变换红外光谱,气相色谱-质谱,核磁共振波谱分析.EHP2的均一分子量为23.6kDa,多分散性窄(Mw/Mn=1.4),EHP2主要由葡萄糖组成（86.4%），半乳糖(11.9%)和甘露糖(1.7%)。EHP2的主要主链是→4）-α-D-Galap-（1→4）-α-D-Glcp-（1→，支链是α-D-Glcp-（1→。通过1,1-二苯基-2-吡啶酰肼（DPPH）和超氧阴离子自由基清除试验评估了EHP2的抗氧化活性，和抗氧化酶活性(SOD，GSH和MDA）在人脐静脉内皮细胞（HUVECs）中测定。EHP2对DPPH和超氧化物自由基清除剂的潜在清除作用低于抗坏血酸，在HUVEC中，它导致SOD和GSH活性增加，MDA水平降低。本研究首次描述了一种具有潜在抗氧化活性的喜马拉雅多糖化合物。
