Abstract：
Aflatoxin B1 (AFB1) is a common mycotoxin that is of significant global concern due to its impact on food safety. Herein, we innovatively develop a sensing platform to detect AFB1 based on evaporation of surfactant solutions on the hydrophobic surface, resulting in dried patterns with varied sizes. The surfactant CTAB solution produces a relatively large dried pattern due to the surface wetting. However, the reduction in the dried pattern size is found when the mixture of CTAB and AFB1 aptamer is tested, because the formation of CTAB/aptamer complex. Moreover, the dried pattern size of the mixture of CTAB, aptamer, and AFB1 increases due to the specific binding of AFB1 to its aptamer. Using this innovative strategy, the AFB1 detection can be fulfilled with a detection limit of 0.77 pg/mL. As a simple, convenient, inexpensive, and label-free method, the surfactant-mediated surface droplet evaporation-based biosensor is very promising for various potential applications.
摘要：
黄曲霉毒素B1（AFB1）是一种常见的真菌毒素，由于其对食品安全的影响而受到全球关注。在这里，我们创新性地开发了一个传感平台来检测AFB1基于表面活性剂溶液在疏水表面的蒸发，导致不同尺寸的干燥图案。表面活性剂CTAB溶液由于表面润湿而产生相对大的干燥图案。然而,当测试CTAB和AFB1适体的混合物时，发现干燥图案尺寸的减小，因为形成CTAB/适配体复合物。此外，CTAB混合物的干燥图案尺寸，适体,并且AFB1由于AFB1与其适体的特异性结合而增加。利用这一创新战略，AFB1检测可以满足0.77pg/mL的检测限。作为一个简单的，方便,便宜,和无标签方法，表面活性剂介导的基于表面液滴蒸发的生物传感器在各种潜在应用中非常有前途。
