Abstract：
In this study, the first nanomaterial-supported molecularly imprinted polymer (MIP)-based electrochemical approach was proposed to achieve the successful detection of cefdinir (CFD). Here, p-amino benzoic acid (p-ABA) was used as the monomer and the photopolymerization method was chosen to form MIP on a glassy carbon electrode (GCE). ZnO nanoparticles (ZnO NPs) were added to the MIP sensor to increase sensitivity and create high porosity. Through the use of cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS), characterization investigations confirmed the alterations at each stage of the MIP production process. Electrochemical (cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS)) and scanning electron microscopy (SEM) methods were used for study the characterization studies of the MIP-based nanocomposite sensor. The measurement of MIP parameters, such as the addition of nanoparticles, the removal procedure, the rebinding period, the monomer ratio, etc., was done using the differential pulse voltammetry (DPV). The findings showed that when ZnO NPs were added, the signal was three times higher than when MIPs were used alone. Under the optimized conditions, CFD/4-ABA@ZnONPs/MIP/GCE showed a linear response in the concentration range between 7.5 pM and 100 pM with LOD and LOQ values of 2.06 pM and 6.86 pM, respectively. Anions, cations, and substances including uric acid, ascorbic acid, paracetamol, and dopamine were all used in the selectivity test. In addition, the imprinting factor (IF) study was carried out using compounds such as cefuroxime, cefazolin, cefixime, ceftazidime, and ceftriaxone, which have structural similarities with CFD, as well as impurities such as thiazolylacetyl glycine oxime (IMP-A), thiazolylacetyl glycine oxime acetal (IMP-B), and cefdinir lactone (IMP-E). The results showed that the proposed sensor was selective for CFD, as evidenced by the relative IF values of these impurities. The recovery studies of CFD were successfully applied to tablet dosage form samples, and the developed sensor demonstrated significant sensitivity and selectivity for rapid detection of CFD in tablet dosage form.
摘要：
在这项研究中,提出了第一种基于纳米材料支持的分子印迹聚合物（MIP）的电化学方法，以成功检测头孢地尼（CFD）。这里,使用对氨基苯甲酸（p-ABA）作为单体，并选择光聚合方法在玻碳电极（GCE）上形成MIP。将ZnO纳米颗粒(ZnONPs)添加到MIP传感器中以增加灵敏度并产生高孔隙率。通过使用循环伏安法（CV）和电化学阻抗谱（EIS），表征调查证实了MIP生产过程每个阶段的变化。电化学（循环伏安法（CV）和电化学阻抗谱（EIS））和扫描电子显微镜（SEM）方法用于研究基于MIP的纳米复合传感器的表征研究。MIP参数的测量，比如添加纳米粒子，拆卸程序,重新绑定期，单体比例，等。,使用差分脉冲伏安法(DPV)完成。研究结果表明，当添加ZnONPs时，该信号是单独使用MIP时的3倍.在优化条件下,CFD/4-ABA@ZnONPs/MIP/GCE在7.5pM至100pM的浓度范围内显示出线性响应，LOD和LOQ值为2.06pM和6.86pM，分别。阴离子,阳离子，和物质包括尿酸，抗坏血酸,扑热息痛,和多巴胺都用于选择性测试。此外,印迹因子（IF）研究使用头孢呋辛等化合物进行，头孢唑啉,头孢克肟,头孢他啶,和头孢曲松,与CFD结构相似，以及诸如噻唑基乙酰基甘氨酸肟（IMP-A）之类的杂质，噻唑基乙酰基甘氨酸肟缩醛（IMP-B），和头孢地尼内酯(IMP-E)。结果表明，所提出的传感器具有选择性的CFD，这些杂质的相对IF值证明了这一点。CFD的回收率研究成功地应用于片剂剂型样品，所开发的传感器对片剂剂型中CFD的快速检测具有显著的灵敏度和选择性。
