Abstract：
Sericulture has become widespread globally, and the utilization of artificial diets produces a substantial quantity of silkworm excrement. Although silkworm excrement can be composted for environmentally friendly disposal, the potential utility of the resulting compost remains underexplored. The aim of this study was to assess the quality of this unique compost and screen for eco-beneficial microbes, providing a new perspective on microbial research in waste management, especially in sustainable agriculture. The low-concentration compost application exhibited a greater plant growth-promoting effect, which was attributed to an appropriate nutritional value (N, P, K, and dissolved organic matter) and the presence of plant growth-promoting bacteria (PGPB) within the compost. Encouraged by the \"One Health\" concept, the eco-benefits of potent PGPB, namely, Klebsiella pneumoniae and Bacillus licheniformis, in sericulture were further evaluated. For plants, K. pneumoniae and B. licheniformis increased plant weight by 152.44 % and 130.91 %, respectively. We also found that even a simple synthetic community composed of the two bacteria performed better than any single bacterium. For animals, K. pneumoniae significantly increased the silkworm (Qiufeng × Baiyu strain) cocoon shell weight by 111.94 %, which could increase sericulture profitability. We also elucidated the mechanism by which K. pneumoniae assisted silkworms in degrading tannic acid, a common plant-derived antifeedant, thereby increasing silkworm feed efficiency. Overall, these findings provide the first data revealing multiple beneficial interactions among silkworm excrement-derived microbes, plants, and animals, highlighting the importance of focusing on microbes in sustainable agriculture.
摘要：
蚕桑已在全球范围内普及,人工饮食的利用会产生大量的蚕粪。虽然蚕粪可以堆肥环保处理，由此产生的堆肥的潜在效用仍未得到充分开发。这项研究的目的是评估这种独特的堆肥的质量和筛选生态有益的微生物，为废物管理中的微生物研究提供了新的视角，尤其是可持续农业。低浓度堆肥施用表现出更大的植物生长促进作用,这归因于适当的营养价值(N，P,K,和溶解的有机物）以及堆肥中植物生长促进细菌（PGPB）的存在。受到“一个健康”概念的鼓舞，有效的PGPB的生态效益，即,肺炎克雷伯菌和地衣芽孢杆菌，在养蚕方面进行了进一步评价。对于植物来说,肺炎克雷伯菌和地衣芽孢杆菌使植物重量增加152.44%和130.91%，分别。我们还发现，即使是由两种细菌组成的简单合成群落也比任何单一细菌表现更好。对于动物来说,肺炎克雷伯菌使家蚕(秋风×白玉株)茧壳重显著增加111.94%,这可以提高蚕桑的盈利能力。我们还阐明了肺炎克雷伯菌辅助蚕降解单宁酸的机制，一种常见的植物来源的拒食剂，从而提高蚕食效率。总的来说,这些发现提供了第一个数据，揭示了蚕粪便来源的微生物之间的多种有益相互作用，植物,和动物,强调在可持续农业中关注微生物的重要性。
