PDF(Pubmed)
Abstract：
While the ecological role that Trichodesmium sp. play in nitrogen fixation has been widely studied, little information is available on potential specialized metabolites that are associated with blooms and standing stock Trichodesmium colonies. While a collection of biological material from a T. thiebautii bloom event from North Padre Island, Texas, in 2014 indicated that this species was a prolific producer of chlorinated specialized metabolites, additional spatial and temporal resolution was needed. We have completed these metabolite comparison studies, detailed in the current report, utilizing LC-MS/MS-based molecular networking to visualize and annotate the specialized metabolite composition of these Trichodesmium blooms and colonies in the Gulf of Mexico (GoM) and other waters. Our results showed that T. thiebautii blooms and colonies found in the GoM have a remarkably consistent specialized metabolome. Additionally, we isolated and characterized one new macrocyclic compound from T. thiebautii, trichothilone A (1), which was also detected in three independent cultures of T. erythraeum. Genome mining identified genes predicted to synthesize certain functional groups in the T. thiebautii metabolites. These results provoke intriguing questions of how these specialized metabolites affect Trichodesmium ecophysiology, symbioses with marine invertebrates, and niche development in the global oligotrophic ocean.
摘要：
而Trichodesmiumsp.的生态作用。固氮的作用已经被广泛研究，关于潜在的专门代谢产物的信息很少，这些代谢产物与水华和常备的Trichodesmium菌落有关。来自北帕德里岛的T.thiebautii开花事件的生物材料，德州,2014年表明该物种是氯化特殊代谢物的多产生产者，需要额外的空间和时间分辨率。我们已经完成了这些代谢物比较研究，在当前报告中详细介绍，利用基于LC-MS/MS的分子网络来可视化和注释墨西哥湾（GoM）和其他水域中这些Trichodesmium花和菌落的特殊代谢物组成。我们的结果表明，在GoM中发现的T.thiebautii花朵和菌落具有非常一致的专门代谢组。此外,我们从T.thiebautii中分离并表征了一种新的大环化合物，曲妥霉素A(1),这也在三个独立的红衣杆菌培养物中检测到。基因组挖掘确定了预测合成T.thiebautii代谢物中某些功能基团的基因。这些结果引发了一些有趣的问题，即这些特殊的代谢物如何影响Trichodesmium生理，与海洋无脊椎动物共生，和全球贫营养海洋的生态位发展。
