Abstract：
This study investigated the anti-inflammatory effects of Pediococcus acidilactici strains isolated from fermented vegetables on lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. In addition, the probiotic characteristics and safety were evaluated. Our results show that Ped. acidilactici strains possess high survivability in simulated gastrointestinal environments and strong attachment to HT-29 cells. All Ped. acidilactici strains exhibited γ-hemolysis and resistance to gentamicin, kanamycin, and streptomycin, a characteristic commonly observed in lactic acid bacteria. Treatment with Ped. acidilactici inhibited the expression of inducible nitric oxide synthase and cyclooxygenase-2, leading to a subsequent reduction in nitric oxide and prostaglandin E2 production. Furthermore, the strains downregulated interleukin (IL)-1β and IL-6 mRNA levels, ultimately suppressing their production. We demonstrated that Ped. acidilactici strains could modulate the activation of nuclear factor-κB, mitogen-activated protein kinase, and activator protein-1, which are known to regulate inflammatory responses. Consequently, the anti-inflammatory properties of Ped. acidilactici strains in this study support their potential application as therapeutic agents for inflammatory diseases, providing molecular insights into next-generation functional probiotic products.
摘要：
本研究调查了从发酵蔬菜中分离的乳酸片球菌菌株对脂多糖（LPS）刺激的RAW264.7巨噬细胞的抗炎作用。此外,对益生菌特性和安全性进行了评价.我们的结果表明，Ped。酸乳菌株在模拟胃肠道环境中具有很高的生存能力，并且对HT-29细胞具有很强的附着力。所有Ped。酸化菌株表现出γ-溶血和对庆大霉素的抗性，卡那霉素,和链霉素,在乳酸菌中通常观察到的特征。用Ped治疗。酸化乳酸抑制诱导型一氧化氮合酶和环氧合酶2的表达，导致随后一氧化氮和前列腺素E2的产生减少。此外，菌株下调白细胞介素（IL）-1β和IL-6mRNA水平，最终抑制了他们的生产。我们证明了Ped。酸乳菌株可以调节核因子-κB的激活，丝裂原活化蛋白激酶,和激活蛋白-1，这是已知的调节炎症反应。因此,Ped的抗炎特性。本研究中的乳酸菌株支持其作为炎症性疾病治疗剂的潜在应用，为下一代功能性益生菌产品提供分子见解。
