Abstract：
So far, the 2019 novel coronavirus (COVID-19) is spreading widely worldwide. The early diagnosis of infection by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is essential to provide timely treatment and prevent its further spread. Lateral flow assays (LFAs) have the advantages of rapid detection, simple operation, low cost, ease of mass production, and no need for special devices and professional operators, which make them suitable for self-testing at home. This review focuses on the early diagnosis of SARS-CoV-2 infection based on optical LFAs including colorimetric, fluorescent (FL), chemiluminescent (CL), and surface-enhanced Raman scattering (SERS) LFAs for the detection of SARS-CoV-2 antigens and nucleic acids. The types of recognition components, detection modes used for antigen detection, labels employed in different optical LFAs, and strategies to improve the detection sensitivity of LFAs were reviewed. Meanwhile, LFAs coupled with different nucleic acid amplification techniques and CRISPR-Cas systems for the detection of SARS-CoV-2 nucleic acids were summarized. We hope this review provides research mentalities for developing highly sensitive LFAs that can be used in home self-testing for the early diagnosis of SARS-CoV-2 infection.
摘要：
到目前为止,2019年新型冠状病毒(COVID-19)正在全球广泛传播。严重急性呼吸综合征冠状病毒-2（SARS-CoV-2）感染的早期诊断对于提供及时治疗和防止其进一步传播至关重要。侧流测定(LFA)具有快速检测的优点,操作简单,低成本,易于大规模生产，不需要特殊设备和专业操作人员，这使得它们适合在家里进行自我测试。本文综述了基于光学LFA的SARS-CoV-2感染的早期诊断，包括比色法，荧光（FL），化学发光(CL)，和表面增强拉曼散射（SERS）LFA，用于检测SARS-CoV-2抗原和核酸。识别组件的类型，用于抗原检测的检测模式，不同光学LFA中使用的标签，并对提高LFA检测灵敏度的策略进行了综述。同时,总结了LFA与不同的核酸扩增技术以及用于检测SARS-CoV-2核酸的CRISPR-Cas系统。我们希望这篇综述为开发高度敏感的LFA提供研究思路，这些LFA可用于家庭自检以早期诊断SARS-CoV-2感染。
