Abstract：
Soybean [Glycine max (L.) Merr.] is an important legume crop worldwide, which provides abundant plant protein and oil for human beings. Soybean mosaic virus (SMV) can cause serious damage to the yield and quality of soybean, but it is difficult to control SMV with chemicals, breeding SMV-resistant varieties has become the most effective way to control the disease. Therefore, it is important to identify SMV resistance genes from soybean resources and apply them to soybean breeding. In this study, the disease rates (DRs) of 219 soybean accessions to SMV strain SC7 in two environments were investigated. A high-density NJAU 355 K SoySNP array was used for genome-wide association study (GWAS) of DR. A 274 kb region on chromosome 15 (1,110,567 bp to 1,384,173 bp) was repeatedly detected in two environments. Six new significant single nucleotide polymorphisms (SNPs) on chromosome 15 were identified. Four of these six SNPs were located within two candidate genes, Glyma.15G015700 and Glyma.15G015800. The elite haplotype Glyma.15G015700Hap I with low DR exhibited strong resistance to SC7. The expression of Glyma.15G015700 in the SMV-resistant accession increased significantly after inoculation with SC7. Furthermore, most of the proteins predicted to interact with Glyma.15G015700 are heat shock proteins, which have been shown to be related to disease resistance. In summary, new SMV resistance loci and a new candidate gene, Glyma.15G015700, were identified and might be utilized in further soybean disease resistance breeding.
摘要：
大豆[最大大豆(L.）合并。]是全球重要的豆类作物，为人类提供丰富的植物蛋白和油脂。大豆花叶病毒(SMV)会对大豆的产量和品质造成严重危害,但是用化学品很难控制SMV，选育抗SMV品种已成为控制该病最有效的方法。因此,从大豆资源中鉴定SMV抗性基因并将其应用于大豆育种具有重要意义。在这项研究中,研究了219份大豆在两种环境中对SMV菌株SC7的病害率（DRs）。高密度NJAU355KSoySNP阵列用于DR的全基因组关联研究（GWAS）。在两种环境中反复检测到15号染色体上的274kb区域（1,110,567bp至1,384,173bp）。在15号染色体上鉴定出6个新的显著单核苷酸多态性(SNPs)。这六个SNP中有四个位于两个候选基因中，Glyma.15G015700和Glyma.15G015800。具有低DR的优良单倍型Glyma.15G015700HapI表现出对SC7的强抗性。接种SC7后，Glyma.15G015700在SMV抗性登录名中的表达显着增加。此外，大多数预测与Glyma相互作用的蛋白质。15G015700是热休克蛋白，已被证明与抗病性有关。总之,新的SMV抗性基因座和新的候选基因，鉴定了Glyma.15G015700，可用于进一步的大豆抗病育种。
