METHODS: Forty male Kunming (KM) mice were categorized into four groups: the control (CON) group, the lipopolysaccharide stimulation (LPS) group, the 5 mg mL-1 OE intervention (LPS + OE) group, and the 5 mg mL-1 OE supplementation plus mixed antibiotics (LPS + OE + ABX) group.
RESULTS: The results showed that, compared with the OE group, the expression of inflammatory signaling pathway genes was upregulated and gut barrier genes were inhibited in the OE + ABX group. The Fxr receptor was activated and the abundance of Akkermansia was increased after OE supplementation, whereas the effect was reversed in the OE + ABX group. Meanwhile, Fxr was correlated positively with Akkermansia.
CONCLUSIONS: The OE supplementation alleviated the inflammatory response in mice under LPS stimulation, accompanied by changes in gut microbiota and bile acid receptors, whereas the addition of antibiotics caused a disturbance to the gut microbiota in the OE group, thus reducing the effect of OE in alleviating the inflammatory response. © 2024 Society of Chemical Industry.
方法:将40只雄性昆明种(KM)小鼠分为4组:对照组(CON),脂多糖刺激(LPS)组,5mgmL-1OE干预(LPS+OE)组,和5mgmL-1OE补充加混合抗生素(LPSOEABX)组。
结果:结果表明,与OE组相比,OE+ABX组炎症信号通路基因表达上调,肠屏障基因受到抑制。补充OE后,Fxr受体被激活,Akkermansia的丰度增加,而OE+ABX组的效果被逆转。同时,Fxr与Akkermansia呈正相关。
结论:OE补充减轻了LPS刺激下小鼠的炎症反应,伴随着肠道菌群和胆汁酸受体的变化,而抗生素的添加对OE组的肠道微生物群造成了干扰,从而降低OE在减轻炎症反应中的作用。©2024化学工业学会。