Abstract：
BACKGROUND: Okra contains flavonoids and vitamin C as antioxidants and it contains polysaccharides as immunomodulators. Flavonoids regulate the inflammatory response in mice and may be related to gut microbiota. This study therefore aimed to investigate the impact of okra extract (OE) on inflammation in mice and to elucidate its underlying mechanism.
METHODS: Forty male Kunming (KM) mice were categorized into four groups: the control (CON) group, the lipopolysaccharide stimulation (LPS) group, the 5 mg mL-1 OE intervention (LPS + OE) group, and the 5 mg mL-1 OE supplementation plus mixed antibiotics (LPS + OE + ABX) group.
RESULTS: The results showed that, compared with the OE group, the expression of inflammatory signaling pathway genes was upregulated and gut barrier genes were inhibited in the OE + ABX group. The Fxr receptor was activated and the abundance of Akkermansia was increased after OE supplementation, whereas the effect was reversed in the OE + ABX group. Meanwhile, Fxr was correlated positively with Akkermansia.
CONCLUSIONS: The OE supplementation alleviated the inflammatory response in mice under LPS stimulation, accompanied by changes in gut microbiota and bile acid receptors, whereas the addition of antibiotics caused a disturbance to the gut microbiota in the OE group, thus reducing the effect of OE in alleviating the inflammatory response. © 2024 Society of Chemical Industry.
METHODS: Forty male Kunming (KM) mice were categorized into four groups: the control (CON) group, the lipopolysaccharide stimulation (LPS) group, the 5 mg mL-1 OE intervention (LPS + OE) group, and the 5 mg mL-1 OE supplementation plus mixed antibiotics (LPS + OE + ABX) group.
RESULTS: The results showed that, compared with the OE group, the expression of inflammatory signaling pathway genes was upregulated and gut barrier genes were inhibited in the OE + ABX group. The Fxr receptor was activated and the abundance of Akkermansia was increased after OE supplementation, whereas the effect was reversed in the OE + ABX group. Meanwhile, Fxr was correlated positively with Akkermansia.
CONCLUSIONS: The OE supplementation alleviated the inflammatory response in mice under LPS stimulation, accompanied by changes in gut microbiota and bile acid receptors, whereas the addition of antibiotics caused a disturbance to the gut microbiota in the OE group, thus reducing the effect of OE in alleviating the inflammatory response. © 2024 Society of Chemical Industry.
摘要：
背景：秋葵含有类黄酮和维生素C作为抗氧化剂，它含有多糖作为免疫调节剂。黄酮类化合物调节小鼠的炎症反应，可能与肠道菌群有关。因此，本研究旨在研究秋葵提取物（OE）对小鼠炎症的影响并阐明其潜在机制。
方法：将40只雄性昆明种（KM）小鼠分为4组：对照组（CON），脂多糖刺激（LPS）组，5mgmL-1OE干预（LPS+OE）组，和5mgmL-1OE补充加混合抗生素（LPSOEABX）组。
结果：结果表明，与OE组相比，OE+ABX组炎症信号通路基因表达上调,肠屏障基因受到抑制。补充OE后，Fxr受体被激活，Akkermansia的丰度增加，而OE+ABX组的效果被逆转。同时,Fxr与Akkermansia呈正相关。
结论：OE补充减轻了LPS刺激下小鼠的炎症反应，伴随着肠道菌群和胆汁酸受体的变化，而抗生素的添加对OE组的肠道微生物群造成了干扰，从而降低OE在减轻炎症反应中的作用。©2024化学工业学会。
方法：将40只雄性昆明种（KM）小鼠分为4组：对照组（CON），脂多糖刺激（LPS）组，5mgmL-1OE干预（LPS+OE）组，和5mgmL-1OE补充加混合抗生素（LPSOEABX）组。
结果：结果表明，与OE组相比，OE+ABX组炎症信号通路基因表达上调,肠屏障基因受到抑制。补充OE后，Fxr受体被激活，Akkermansia的丰度增加，而OE+ABX组的效果被逆转。同时,Fxr与Akkermansia呈正相关。
结论：OE补充减轻了LPS刺激下小鼠的炎症反应，伴随着肠道菌群和胆汁酸受体的变化，而抗生素的添加对OE组的肠道微生物群造成了干扰，从而降低OE在减轻炎症反应中的作用。©2024化学工业学会。
