UNASSIGNED: Synovium and adipose tissues were concurrently harvested from eight patients with knee osteoarthritis. Suspensions of both synovial and adipose MSCs were examined to identify the presence of microspikes. In addition to this study, the MSC suspensions in four patients were applied to abraded porcine cartilage discs and observed 10 s, 10 min, and 1 h later.
UNASSIGNED: The median percentage of cells exhibiting microspikes was 14% for synovial MSC suspensions and 13% for adipose MSC suspensions; this difference was not statistically significant (n = 8). No notable differences were detected in the number of adherent cells or in the proportion of cells displaying microspikes or pseudopodia. Strong correlations were found between the proportion of cells with pseudopodia and the number of attached cells for both synovial (r = 0.92, n = 12) and adipose (r = 0.86, n = 12) MSCs, with no significant difference in the correlation coefficients between the two groups.
UNASSIGNED: SEM analysis revealed no obvious differences in morphological characteristics during MSC adhesion to cartilage for either synovial or adipose MSCs.
■同时从8名膝骨关节炎患者中采集滑膜和脂肪组织。检查滑膜和脂肪MSC的悬浮液以鉴定微尖峰的存在。除了这项研究,将四名患者的MSC悬浮液应用于磨蚀的猪软骨盘并观察10s,10分钟,1小时后。
表现出微尖峰的细胞的中值百分比对于滑膜MSC悬浮液为14%,对于脂肪MSC悬浮液为13%;该差异在统计学上不显著(n=8)。在贴壁细胞的数量或显示微尖峰或伪足的细胞比例中未检测到显着差异。滑膜(r=0.92,n=12)和脂肪(r=0.86,n=12)MSCs的假足细胞比例与附着细胞数量之间存在很强的相关性,两组相关系数无显著差异。
■SEM分析显示,对于滑膜或脂肪MSC,在MSC粘附至软骨期间的形态特征没有明显差异。