Abstract：
UNASSIGNED: Traditional fermented meat products can be considered a source of bioactive peptides. Cangkuk, a traditional Indonesian fermented beef product is one source of ACE inhibitory peptides. This study aimed to identify ACE-inhibitory peptides from Cangkuk and analyze their antihypertensive effects.
UNASSIGNED: The water-soluble fraction of Cangkuk was fractionated to obtain ACE-inhibitory peptides using an ethanol solvent at several concentrations and solid-phase extraction with an OASIS HLB cartridge followed by purification with RP-HPLC. HPLC-MS was used to identify target peptides, followed by automatic protein sequencer analysis to detect peptide sequences. Antihypertensive effects were analyzed on the water-soluble fraction and synthesized peptides. The animal model was comprised of 14-16-week-old male spontaneously hypertensive rats (SHRs) [~320 g average body weight] with mean systolic blood pressures (SBPs) higher than 190 mm Hg. All oral doses of peptides were 1 mL in volume. Distilled water was used as a control. The antihypertensive activities of the sample and control were observed by measuring the SBP at 0, 2, 4, 6, 8 and 24 h after oral administration.
UNASSIGNED: Two sequences of ACE inhibitory peptides were found, EAPLNPKANR (IC50 value of 44.6 µmol L-1) and IVG (IC50 value of 97.3 µmol L-1). The water-soluble fraction demonstrated an antihypertensive effect on SHRs after oral administration at 100 mg kg-1 body weight, maximally lowering the SBP by 14.9 mm Hg 8 h after administration. The tripeptide IVG showed the highest reduction of SBP, 24.76±2.1 mm Hg 8 h after administration. The decapeptide EAPLNPKANR showed the highest reduction of SBP, 21.0 ±1.9 mm Hg, 8 h after administration. All the samples differed significantly from the control (p<0.01).
UNASSIGNED: Cangkuk has potential as a functional food ingredient acting as an antihypertensive agent.
UNASSIGNED: The water-soluble fraction of Cangkuk was fractionated to obtain ACE-inhibitory peptides using an ethanol solvent at several concentrations and solid-phase extraction with an OASIS HLB cartridge followed by purification with RP-HPLC. HPLC-MS was used to identify target peptides, followed by automatic protein sequencer analysis to detect peptide sequences. Antihypertensive effects were analyzed on the water-soluble fraction and synthesized peptides. The animal model was comprised of 14-16-week-old male spontaneously hypertensive rats (SHRs) [~320 g average body weight] with mean systolic blood pressures (SBPs) higher than 190 mm Hg. All oral doses of peptides were 1 mL in volume. Distilled water was used as a control. The antihypertensive activities of the sample and control were observed by measuring the SBP at 0, 2, 4, 6, 8 and 24 h after oral administration.
UNASSIGNED: Two sequences of ACE inhibitory peptides were found, EAPLNPKANR (IC50 value of 44.6 µmol L-1) and IVG (IC50 value of 97.3 µmol L-1). The water-soluble fraction demonstrated an antihypertensive effect on SHRs after oral administration at 100 mg kg-1 body weight, maximally lowering the SBP by 14.9 mm Hg 8 h after administration. The tripeptide IVG showed the highest reduction of SBP, 24.76±2.1 mm Hg 8 h after administration. The decapeptide EAPLNPKANR showed the highest reduction of SBP, 21.0 ±1.9 mm Hg, 8 h after administration. All the samples differed significantly from the control (p<0.01).
UNASSIGNED: Cangkuk has potential as a functional food ingredient acting as an antihypertensive agent.
摘要：
■传统的发酵肉制品可以被认为是生物活性肽的来源。Cangkuk,传统的印尼发酵牛肉产品是ACE抑制肽的一种来源。本研究旨在鉴定Cangkuk中的ACE抑制肽并分析其降压作用。
使用几种浓度的乙醇溶剂和用OASISHLB柱进行固相萃取,然后用RP-HPLC纯化,对Cangkuk的水溶性级分进行分馏以获得ACE抑制肽。HPLC-MS用于鉴定目标肽，然后进行自动蛋白质测序仪分析以检测肽序列。分析了对水溶性部分和合成肽的抗高血压作用。动物模型由14-16周龄雄性自发性高血压大鼠（SHR）[〜320g平均体重]组成，平均收缩压（SBP）高于190mmHg。肽的所有口服剂量为1mL体积。蒸馏水用作对照。通过在口服给药后0、2、4、6、8和24小时测量SBP来观察样品和对照的抗高血压活性。
■发现了两个ACE抑制肽序列，EAPLNPKANR（IC50值为44.6µmolL-1）和IVG（IC50值为97.3µmolL-1）。口服100mgkg-1体重后，水溶性部分对SHR具有抗高血压作用，给药后8小时最大限度地降低SBP14.9mmHg。三肽IVG显示出最高的SBP降低，给药后8小时24.76±2.1mmHg。十肽EAPLNPKANR表现出最高的SBP降低，21.0±1.9mmHg,给药后8小时。所有样品与对照显著不同(p<0.01)。
■Cangkuk具有作为抗高血压药的功能性食品成分的潜力。
使用几种浓度的乙醇溶剂和用OASISHLB柱进行固相萃取,然后用RP-HPLC纯化,对Cangkuk的水溶性级分进行分馏以获得ACE抑制肽。HPLC-MS用于鉴定目标肽，然后进行自动蛋白质测序仪分析以检测肽序列。分析了对水溶性部分和合成肽的抗高血压作用。动物模型由14-16周龄雄性自发性高血压大鼠（SHR）[〜320g平均体重]组成，平均收缩压（SBP）高于190mmHg。肽的所有口服剂量为1mL体积。蒸馏水用作对照。通过在口服给药后0、2、4、6、8和24小时测量SBP来观察样品和对照的抗高血压活性。
■发现了两个ACE抑制肽序列，EAPLNPKANR（IC50值为44.6µmolL-1）和IVG（IC50值为97.3µmolL-1）。口服100mgkg-1体重后，水溶性部分对SHR具有抗高血压作用，给药后8小时最大限度地降低SBP14.9mmHg。三肽IVG显示出最高的SBP降低，给药后8小时24.76±2.1mmHg。十肽EAPLNPKANR表现出最高的SBP降低，21.0±1.9mmHg,给药后8小时。所有样品与对照显著不同(p<0.01)。
■Cangkuk具有作为抗高血压药的功能性食品成分的潜力。
