Abstract：
The objective of this study was to evaluate the synergistic effect of eggshell-derived nanohydroxyapatite (EnHA) and carboxymethyl chitosan (CMC) in remineralizing artificially induced dentinal lesions. EnHA and CMC were synthesized using simple chemical processes and characterized using FTIR, XRD, HRSEM-EDX, TEM, DLS and TGA/DTA analyses. A total of 64 pre-demineralized coronal dentin specimens were randomly subjected to following treatments (n = 16):artificial saliva (AS), EnHA, CMC, and EnHA-CMC, followed by pH cycling for 7 days. HRSEM-EDX, Vickers-indenter, and micro-Raman analyses were used to assess surface-topography, microhardness, and chemical analysis, respectively. All tested materials demonstrated non-cytotoxicity when assessed on hDPSCs using MTT assay. FTIR, XRD and thermal analyses confirmed the characteristics of both EnHA and CMC. EnHA showed irregular rod-shaped nanoparticles (30-70 nm) with the presence of Ca,P,Na, and Mg ions. Dentin treated with EnHA-CMC exhibited complete tubular occlusion and highest microhardness whereas the AS group revealed the least mineral deposits (p < 0.05). No significant differences were observed between EnHA and CMC groups (p > 0.05). In addition, molecular conformation analysis revealed peak intensities in collagen\'s polypeptide chains in dentin treated with CMC and EnHA-CMC, whereas other groups showed poor collagen stability. The results highlighted that EnHA-CMC aided in rapid and effective biomineralization, suggesting its potential as a therapeutic solution for treating dentin caries.
摘要：
这项研究的目的是评估蛋壳衍生的纳米羟基磷灰石（EnHA）和羧甲基壳聚糖（CMC）在再矿化人工诱导的牙本质病变中的协同作用。使用简单的化学方法合成了EnHA和CMC，并使用FTIR进行了表征，XRD,HRSEM-EDX,TEM,DLS和TGA/DTA分析。总共64个预脱矿质的冠状牙本质标本随机接受以下处理（n=16）：人工唾液（AS），EnHA,CMC,和EnHA-CMC,然后是7天的pH循环。HRSEM-EDX,维氏压头,和显微拉曼分析用于评估表面形貌，显微硬度,和化学分析，分别。当使用MTT测定在hDPSC上评估时,所有测试的材料都表现出非细胞毒性。FTIR,XRD和热分析证实了EnHA和CMC的特征。EnHA在Ca的存在下显示出不规则的棒状纳米颗粒（30-70nm），P,Na,镁离子。用EnHA-CMC处理的牙本质表现出完全的肾小管闭塞和最高的显微硬度，而AS组显示出最少的矿物质沉积（p<0.05）。EnHA和CMC组之间没有观察到显著差异(p>0.05)。此外,分子构象分析显示用CMC和EnHA-CMC处理的牙本质中胶原多肽链的峰强度，而其他组显示较差的胶原蛋白稳定性。结果表明，EnHA-CMC有助于快速有效的生物矿化，表明其作为治疗牙本质龋齿的治疗方案的潜力。
