Abstract：
Currently, evidence from observational studies suggests dietary fiber intake may be associated with decreased risk of food allergy. As a type of dietary fiber, resistant starch was also widely reported to possess anti-allergic properties. However, there is a relative paucity of studies assessing the influence of resistant starch types on their anti-allergic activity and its possible underlying mechanisms. In the current study, the anti-allergic effects of RS3-type (retrograded starch), RS4-type (chemically modified starch, cross-bonded), and RS5-type (starch-palmitic acid complex) of lotus seed resistant starch were evaluated in the OVA (100 mg/kg)-induced food allergic mice model. The results showed that oral administration of RS3 or RS4 lotus seed resistant starch (0.3 g/100 g b.w.) for 25 days significantly improved adverse symptoms of food allergy such as weight loss, increases in allergy symptom score and diarrhea rate; with significant reduction of serum specific antibody IgE, TNF-α, IL-4 levels and improved Th1/Th2 balance being observed. The mechanism may involve the regulation of lotus seed resistant starch on intestinal flora and the metabolites short-chain fatty acids and bile acids. Taken together, the findings may enhance understanding towards ameliorative effects of resistant starch on food allergy, and offer valuable insights for the exploration of novel anti-allergic bioactive compounds.
摘要：
目前,观察性研究的证据表明，膳食纤维的摄入可能与食物过敏风险的降低有关。作为一种膳食纤维，抗性淀粉也被广泛报道具有抗过敏特性。然而,评估抗性淀粉类型对其抗过敏活性的影响及其可能的潜在机制的研究相对较少。在目前的研究中,RS3型（逆行淀粉）的抗过敏作用，RS4型(化学改性淀粉，交叉结合)，在OVA（100mg/kg）诱导的食物过敏小鼠模型中评估了莲子抗性淀粉的RS5型（淀粉-棕榈酸复合物）。结果表明，口服RS3或RS4莲子抗性淀粉（0.3g/100gb.w.）25天显着改善了食物过敏的不良症状，例如体重减轻，过敏症状评分和腹泻率增加；血清特异性抗体IgE显著降低，TNF-α,观察到IL-4水平和改善的Th1/Th2平衡。其机制可能涉及莲子抗性淀粉对肠道菌群及其代谢产物短链脂肪酸和胆汁酸的调节作用。一起来看,这些发现可能会增强对抗性淀粉对食物过敏的改善作用的理解，并为探索新型抗过敏生物活性化合物提供有价值的见解。
