Abstract：
Plant growth-promoting endophytes (PGPE) can effectively regulate plant growth and metabolism. The regulation is modulated by metabolic signals, and the resulting metabolites can have considerable effects on the plant yield and quality. Here, tissue culture Houttuynia cordata Thunb., was inoculated with Rhizobium sp. (BH46) to determine the effect of BH46 on H. cordata growth and metabolism, and elucidate associated regulatory mechanisms. The results revealed that BH46 metabolized indole-3-acetic acid and induced 1-aminocyclopropane-1-carboxylate deaminase to decrease ethylene metabolism. Host peroxidase synthesis MPK3/MPK6 genes were significantly downregulated, whereas eight genes associated with auxins, cytokinins, abscisic acid, jasmonic acid, and antioxidant enzymes were significantly upregulated. Eight genes associated with flavonoid biosynthesis were significantly upregulated, with the CPY75B1 gene regulating the production of rutin and quercitrin and the HCT gene directly regulating the production of chlorogenic acid. Therefore, BH46 influences metabolic signals in H. cordata to modulate its growth and metabolism, in turn, enhancing yield and quality of H. cordata.
摘要：
植物促生长内生菌(PGPE)能有效调节植物生长和代谢。这种调节是由代谢信号调节的，产生的代谢产物会对植物的产量和品质产生相当大的影响。这里,组织培养鱼腥草。,接种根瘤菌。（BH46）确定BH46对H.cordata生长和代谢的影响，并阐明相关的调控机制。结果表明，BH46代谢吲哚-3-乙酸并诱导1-氨基环丙烷-1-羧酸脱氨酶降低乙烯代谢。宿主过氧化物酶合成MPK3/MPK6基因显著下调，而8个与生长素相关的基因，细胞分裂素,脱落酸，茉莉酸,和抗氧化酶显著上调。8个与类黄酮生物合成相关的基因显著上调,CPY75B1基因调控芦丁和槲皮苷的生产,HCT基因直接调控绿原酸的生产。因此,BH46影响H.cordata中的代谢信号以调节其生长和代谢，反过来,提高紫菜的产量和质量。
