Abstract：
Reliable predictions for the route and accumulation of nanotherapeutics in vivo are limited by the huge gap between the 2D in vitro assays used for drug screening and the 3D physiological in vivo environment. While developing a standard 3D in vitro model for screening nanotherapeutics remains challenging, multi-cellular tumor spheroids (MCTS) are a promising in vitro model for such screening. Here, we present a straightforward and flexible 3D-model microsystem made out of agarose-based micro-wells, which enables the formation of hundreds of reproducible spheroids in a single pipetting. Immunostaining and fluorescent imaging, including live high-resolution optical microscopy, can be done in situ without manipulating spheroids.
摘要：
对纳米治疗剂在体内的途径和积累的可靠预测受到用于药物筛选的2D体外测定与3D生理体内环境之间的巨大差距的限制。虽然开发用于筛选纳米疗法的标准3D体外模型仍然具有挑战性，多细胞肿瘤球状体（MCTS）是一种有前途的体外筛选模型。这里,我们提出了一个简单而灵活的3D模型微系统，由基于琼脂糖的微孔制成，这使得在单次移液中形成数百个可重复的球体。免疫染色和荧光成像，包括实时高分辨率光学显微镜，可以在原位完成而无需操纵球体。
