Abstract：
Circulating tumor cells (CTCs) isolated directly from whole blood opens new perspectives for cancer monitoring and the development of personalized treatments. However, due to their rarity among the multitude of blood cells, it remains a challenge to recover them alive with high level of purity, i.e., with few remaining white blood cells, and in a time frame compatible with the clinical context. Microfluidic chips have emerged as promising tools to address these challenges. We propose a two-step workflow including a pre-enrichment step, performed by a size-based pre-enrichment system, and a purification step, performed by an immunomagnetic chip. Here, we describe the protocol for the fabrication of the immunomagnetic microchip, the preparation of the sample, and the procedure for injection into the microchip allowing the sorting of the CTCs.
摘要：
直接从全血分离的循环肿瘤细胞（CTC）为癌症监测和个性化治疗的发展开辟了新的前景。然而,由于它们在众多血细胞中的稀有性，以高纯度恢复它们仍然是一个挑战，即，剩下的白细胞很少，并且在与临床背景兼容的时间范围内。微流体芯片已经成为解决这些挑战的有希望的工具。我们提出了一个两步工作流程，包括一个预富集步骤，由基于大小的预浓缩系统执行，和纯化步骤，由免疫磁性芯片执行。这里,我们描述了制造免疫磁性微芯片的协议，样品的制备，以及用于注射到微芯片中的程序，允许对CTC进行分选。
