Abstract：
OBJECTIVE: The aim of this study is the evaluation effect of nanoliposome-loaded Mito-Tempo on sperm parameters during human sperm cryopreservation.
METHODS: Semen samples of 50 Asthenoteratozoospermia men (random) were collected. Sperm parameters were analyzed based on World Health Organization (WHO, 2010) criteria (2021) and each sample was divided into 5 groups (E1-E5). E1 (control group): the sperm was cryopreserved without nanoliposome, and Mito-Tempo. E2: sperm cryopreservation with Mito-Tempo-loaded nanoliposome (Mito-Tempo 0.1 mM) + freezing medium. E3: sperm cryopreservation with Mito-Tempo-loaded nanoliposome (Mito-Tempo 0.2 mM) + freezing medium. E4: in this group, the cryopreservation sperm with Mito-Tempo 0.3 mM + freezing medium. E5: the cryopreservation sperm with Mito-Tempo 0.2 mM + freezing medium.
RESULTS: The result of this study indicated that sperm parameters and total antioxidant capacity (TAC) significantly increase in E3 and E4 groups, compared to E1, E2, and E5 groups respectively (P < 0.05). The percentage of abnormal morphology, DNA fragmentation index (DFI), malondialdehyde (MDA), and the levels of ROS significantly decrease in E3 and E4 groups, compared to E1, E2, and E5 groups (P < 0.05). In addition, the sperm parameters and stress oxidative factors significantly improve in E3 group compared to other groups (P < 0.05).
CONCLUSIONS: In conclusion, the combination of Mito-Tempo with nanoliposome due to its ability to cooperate with lipid layers may lead to significant performance in reducing oxidative stress damage and increasing the quality of sperm parameters.
METHODS: Semen samples of 50 Asthenoteratozoospermia men (random) were collected. Sperm parameters were analyzed based on World Health Organization (WHO, 2010) criteria (2021) and each sample was divided into 5 groups (E1-E5). E1 (control group): the sperm was cryopreserved without nanoliposome, and Mito-Tempo. E2: sperm cryopreservation with Mito-Tempo-loaded nanoliposome (Mito-Tempo 0.1 mM) + freezing medium. E3: sperm cryopreservation with Mito-Tempo-loaded nanoliposome (Mito-Tempo 0.2 mM) + freezing medium. E4: in this group, the cryopreservation sperm with Mito-Tempo 0.3 mM + freezing medium. E5: the cryopreservation sperm with Mito-Tempo 0.2 mM + freezing medium.
RESULTS: The result of this study indicated that sperm parameters and total antioxidant capacity (TAC) significantly increase in E3 and E4 groups, compared to E1, E2, and E5 groups respectively (P < 0.05). The percentage of abnormal morphology, DNA fragmentation index (DFI), malondialdehyde (MDA), and the levels of ROS significantly decrease in E3 and E4 groups, compared to E1, E2, and E5 groups (P < 0.05). In addition, the sperm parameters and stress oxidative factors significantly improve in E3 group compared to other groups (P < 0.05).
CONCLUSIONS: In conclusion, the combination of Mito-Tempo with nanoliposome due to its ability to cooperate with lipid layers may lead to significant performance in reducing oxidative stress damage and increasing the quality of sperm parameters.
摘要：
目的：本研究的目的是评估纳米脂质体负载的Mito-Tempo在人精子冷冻保存过程中对精子参数的影响。
方法：收集50例无性生殖精子症男性（随机）的精液样本。精子参数根据世界卫生组织(WHO，2010)标准(2021)，每个样本分为5组(E1-E5)。E1（对照组）：精子冷冻保存，不含纳米脂质体，还有Mito-Tempo.E2:用Mito-Tempo负载的纳米脂质体(Mito-Tempo0.1mM)+冷冻培养基冷冻保存精子。E3:用Mito-Tempo负载的纳米脂质体(Mito-Tempo0.2mM)+冷冻培养基冷冻保存精子。E4：在这个组中，用Mito-Tempo0.3mM+冷冻培养基冷冻保存精子。E5:具有Mito-Tempo0.2mM+冷冻培养基的冷冻保存精子。
结果：这项研究的结果表明，E3和E4组的精子参数和总抗氧化能力（TAC）显着增加，E1、E2、E5组比较差异有统计学意义(P<0.05)。形态异常的百分比，DNA片段化指数(DFI),丙二醛（MDA），E3和E4组的ROS水平显着降低，E1、E2、E5组比较(P<0.05)。此外,与其他组相比，E3组的精子参数和应激氧化因子显着改善（P<0.05）。
结论：结论：Mito-Tempo与纳米脂质体的组合由于其与脂质层合作的能力而可能导致在减少氧化应激损伤和提高精子参数质量方面的显着表现。
方法：收集50例无性生殖精子症男性（随机）的精液样本。精子参数根据世界卫生组织(WHO，2010)标准(2021)，每个样本分为5组(E1-E5)。E1（对照组）：精子冷冻保存，不含纳米脂质体，还有Mito-Tempo.E2:用Mito-Tempo负载的纳米脂质体(Mito-Tempo0.1mM)+冷冻培养基冷冻保存精子。E3:用Mito-Tempo负载的纳米脂质体(Mito-Tempo0.2mM)+冷冻培养基冷冻保存精子。E4：在这个组中，用Mito-Tempo0.3mM+冷冻培养基冷冻保存精子。E5:具有Mito-Tempo0.2mM+冷冻培养基的冷冻保存精子。
结果：这项研究的结果表明，E3和E4组的精子参数和总抗氧化能力（TAC）显着增加，E1、E2、E5组比较差异有统计学意义(P<0.05)。形态异常的百分比，DNA片段化指数(DFI),丙二醛（MDA），E3和E4组的ROS水平显着降低，E1、E2、E5组比较(P<0.05)。此外,与其他组相比，E3组的精子参数和应激氧化因子显着改善（P<0.05）。
结论：结论：Mito-Tempo与纳米脂质体的组合由于其与脂质层合作的能力而可能导致在减少氧化应激损伤和提高精子参数质量方面的显着表现。
