Abstract：
The Stat (signal transducer and activator of transcription) gene family plays a vital role in regulating immunity and the processes of cellular proliferation, differentiation, and apoptosis across diverse organisms. Although the functions of Stat genes in immunity have been extensively documented in many mammals, limited data are available for reptiles. We used phylogenetic analysis to identify eight putative members of the Stat family (Stat1-1, Stat1-2, Stat2, Stat3, Stat4, Stat5b, Stat6-1, and Stat6-2) within the genome of M. reevesii, a freshwater turtle found in East Asia. Sequence analysis showed that the Stat genes contain four conserved structural domains protein interaction domain, coiled-coil domain, DNA-binding domain, and Src homology domain 2. In addition, Stat1, Stat2, and Stat6 contain TAZ2bind, Apolipo_F, and TALPID3 structural domains. The mRNA levels of Stat genes were upregulated in spleen tissues at 4, 8, 12, and 16 h after administration of lipopolysaccharide, a potent activator of the immune system. Stat5b expression at 12-h LPS post-injection exhibited the most substantial difference from the control. The expression of Stat5b in spleen tissue cellular was verified by immunofluorescence. These results suggest that Stat5b plays a role in the immune response of M. reevesii and may prove to be as a positive marker of an immune response in future studies.
摘要：
Stat（信号转导和转录激活因子）基因家族在调节免疫和细胞增殖过程中起着至关重要的作用，分化,以及跨不同生物体的细胞凋亡。尽管Stat基因在免疫中的功能已经在许多哺乳动物中得到了广泛的证明,爬行动物的可用数据有限。我们使用系统发育分析来鉴定Stat家族的八个推定成员（Stat1-1，Stat1-2，Stat2，Stat3，Stat4，Stat5b，Stat6-1和Stat6-2）在M.reevesii的基因组中，一种在东亚发现的淡水龟。序列分析表明,Stat基因含有4个保守结构域蛋白质相互作用结构域,卷曲螺旋结构域，DNA结合域,和Src同源结构域2.此外,Stat1、Stat2和Stat6包含TAZ2bind，Apolipo_F,和TALPID3结构域。脂多糖给药后4、8、12和16小时，脾组织中Stat基因的mRNA水平上调，免疫系统的有效激活剂。在注射后12小时LPS时的Stat5b表达表现出与对照的最显著差异。免疫荧光法证实Stat5b在脾组织细胞中的表达。这些结果表明，Stat5b在瑞维氏杆菌的免疫反应中起作用，并且可能在未来的研究中被证明是免疫反应的阳性标志物。
