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Abstract：
Aim The study aims to evaluate the antibacterial properties of ethanolic extracts from Clitoria ternatea and Camellia sinensis against pathogens causing UTI, wound pathogens, and other clinical bacterial infections and their cytotoxic effects using the brine shrimp lethality assay (BSLA). Methods Ethanolic extracts of C. ternatea and C. sinensis were prepared, and their antibacterial activity was tested against Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Enterococcus faecalis using the well diffusion method. The cytotoxicity was assessed through the BSLA, determining the LC50 values for each extract. Results The formulation of both plant extracts exhibited significant antibacterial activity against UTI pathogens, and wound pathogen bacteria showed higher efficacy compared to other studies. The BSLA revealed a dose-dependent increase in toxicity, with C. ternatea extracts demonstrating higher cytotoxicity than C. sinensis. Conclusion The ethanolic extracts of C. ternatea and C. sinensis possess antibacterial properties against UTI-causing bacteria and show cytotoxic effects in a brine shrimp model. These findings suggest the potential of these plants for developing alternative treatments for UTI. However, further research is necessary to fully understand their safety and efficacy in human subjects.
摘要：
目的评价丁香和茶树乙醇提取物对引起UTI的病原菌的抗菌性能。伤口病原体，和其他临床细菌感染及其使用盐水虾致死性测定法（BSLA）的细胞毒性作用。方法制备三七和华七的乙醇提取物，它们的抗菌活性被测试对金黄色葡萄球菌，大肠杆菌,肺炎克雷伯菌,铜绿假单胞菌,和粪肠球菌使用井扩散法。通过BSLA评估细胞毒性，确定每个提取物的LC50值。结果两种植物提取物的配方对UTI病原体均具有明显的抗菌活性，与其他研究相比，伤口病原体细菌显示出更高的疗效。BSLA揭示了毒性的剂量依赖性增加，C.ternatea提取物显示出比C.sinensis更高的细胞毒性。结论三黄曲霉和中华黄曲霉的乙醇提取物对引起UTI的细菌具有抗菌性能，并在盐水虾模型中显示出细胞毒性作用。这些发现表明这些植物开发UTI替代治疗的潜力。然而,需要进一步研究以充分了解其在人类受试者中的安全性和有效性。
