UNASSIGNED: PNAG was detected on the surface of C. acnes cells by fluorescence confocal microscopy using the antigen-specific human IgG1 monoclonal antibody F598. PNAG was detected in C. acnes biofilms by measuring the ability of the PNAG-specific glycosidase dispersin B to inhibit biofilm formation and sensitize biofilms to biocide killing.
UNASSIGNED: Monoclonal antibody F598 bound to the surface of C. acnes cells. Dispersin B inhibited attachment of C. acnes cells to polystyrene rods, inhibited biofilm formation by C. acnes in glass and polypropylene tubes, and sensitized C. acnes biofilms to killing by benzoyl peroxide and tetracycline.
UNASSIGNED: C. acnes produces PNAG, and PNAG contributes to C. acnes biofilm formation and biocide resistance in vitro. PNAG may play a role in C. acnes skin colonization, biocide resistance, and virulence in vivo.
■使用抗原特异性人IgG1单克隆抗体F598通过荧光共聚焦显微镜在痤疮梭菌细胞表面上检测到PNAG。通过测量PNAG特异性糖苷酶分散剂B抑制生物膜形成和使生物膜对杀生物剂杀伤敏感的能力,在痤疮梭菌生物膜中检测到PNAG。
■单克隆抗体F598与痤疮梭菌细胞的表面结合。分散素B抑制痤疮杆菌细胞附着于聚苯乙烯棒,抑制玻璃和聚丙烯管中痤疮杆菌生物膜的形成,并使痤疮杆菌生物膜对过氧化苯甲酰和四环素的杀伤敏感。
■C.痤疮产生PNAG,和PNAG有助于痤疮梭菌生物膜形成和体外抗微生物剂。PNAG可能在痤疮梭菌皮肤定植中起作用,抗杀菌剂,和体内毒力。