Abstract：
OBJECTIVE: In previous studies, it was demonstrated that co-culturing Clostridium pasteurianum and Geobacter sulfurreducens triggers a metabolic shift in the former during glycerol fermentation. This shift, attributed to interspecies electron transfer and the exchange of other molecules, enhances the production of 1,3-propanediol at the expense of the butanol pathway. The aim of this investigation is to examine the impact of fumarate, a soluble compound usually used as an electron acceptor for G. sulfurreducens, in the metabolic shift previously described in C. pasteurianum.
RESULTS: Experiments were conducted by adding along with glycerol, acetate, and different quantities of fumarate in co-cultures of G. sulfurreducens and C. pasteurianum. A metabolic shift was exhibited in all the co-culture conditions. This shift was more pronounced at higher fumarate concentrations. Additionally, we observed G. sulfurreducens growing even in the absence of fumarate and utilizing small amounts of this compound as an electron donor rather than an electron acceptor in the co-cultures with high fumarate addition.
CONCLUSIONS: This study provided evidence that interspecies electron transfer continues to occur in the presence of a soluble electron acceptor, and the metabolic shift can be enhanced by promoting the growth of G. sulfurreducens.
RESULTS: Experiments were conducted by adding along with glycerol, acetate, and different quantities of fumarate in co-cultures of G. sulfurreducens and C. pasteurianum. A metabolic shift was exhibited in all the co-culture conditions. This shift was more pronounced at higher fumarate concentrations. Additionally, we observed G. sulfurreducens growing even in the absence of fumarate and utilizing small amounts of this compound as an electron donor rather than an electron acceptor in the co-cultures with high fumarate addition.
CONCLUSIONS: This study provided evidence that interspecies electron transfer continues to occur in the presence of a soluble electron acceptor, and the metabolic shift can be enhanced by promoting the growth of G. sulfurreducens.
摘要：
目的：在以往的研究中，已证明，在甘油发酵过程中，共培养巴氏梭菌和硫化焦菌可触发前者的代谢变化。这个转变,归因于种间电子转移和其他分子的交换，以丁醇途径为代价增强1,3-丙二醇的生产。这项调查的目的是检查富马酸盐的影响，一种可溶性化合物，通常用作G.硫还原的电子受体，在先前在巴氏杆菌中描述的代谢转变中。
结果：实验是通过与甘油一起添加，硫酸还原菌和巴氏杆菌共培养物中的乙酸盐和不同量的富马酸盐。在所有共培养条件下都表现出代谢转移。这种变化在较高的富马酸盐浓度下更明显。此外,我们观察到，即使在不存在富马酸盐的情况下，硫还原菌也在高富马酸盐添加量的共培养物中使用少量该化合物作为电子供体而不是电子受体。
结论：这项研究提供了证据，表明在可溶性电子受体的存在下，种间电子转移继续发生，通过促进硫还原G的生长可以增强代谢转变。
结果：实验是通过与甘油一起添加，硫酸还原菌和巴氏杆菌共培养物中的乙酸盐和不同量的富马酸盐。在所有共培养条件下都表现出代谢转移。这种变化在较高的富马酸盐浓度下更明显。此外,我们观察到，即使在不存在富马酸盐的情况下，硫还原菌也在高富马酸盐添加量的共培养物中使用少量该化合物作为电子供体而不是电子受体。
结论：这项研究提供了证据，表明在可溶性电子受体的存在下，种间电子转移继续发生，通过促进硫还原G的生长可以增强代谢转变。
