RESULTS: Experiments were conducted by adding along with glycerol, acetate, and different quantities of fumarate in co-cultures of G. sulfurreducens and C. pasteurianum. A metabolic shift was exhibited in all the co-culture conditions. This shift was more pronounced at higher fumarate concentrations. Additionally, we observed G. sulfurreducens growing even in the absence of fumarate and utilizing small amounts of this compound as an electron donor rather than an electron acceptor in the co-cultures with high fumarate addition.
CONCLUSIONS: This study provided evidence that interspecies electron transfer continues to occur in the presence of a soluble electron acceptor, and the metabolic shift can be enhanced by promoting the growth of G. sulfurreducens.
结果:实验是通过与甘油一起添加,硫酸还原菌和巴氏杆菌共培养物中的乙酸盐和不同量的富马酸盐。在所有共培养条件下都表现出代谢转移。这种变化在较高的富马酸盐浓度下更明显。此外,我们观察到,即使在不存在富马酸盐的情况下,硫还原菌也在高富马酸盐添加量的共培养物中使用少量该化合物作为电子供体而不是电子受体。
结论:这项研究提供了证据,表明在可溶性电子受体的存在下,种间电子转移继续发生,通过促进硫还原G的生长可以增强代谢转变。