Abstract：
Pigmentation genes expressed in skin, body muscle and tail of Thai-flag compared with Blue, White and Red varieties of Siamese fighting fish Betta splendens were identified. In total, 22,919 new unigenes were found. Pearson correlation and PCA analysis revealed that expression profiles of genes in muscle, skin and tail across solid color variety were similar. In contrast, those in skin and red tail part of Thai-flag were closely related but they showed different expression profiles with the white tail part. Moreover, 21,347-64,965 SNPs were identified in exonic regions of identified genes. In total, 28,899 genes were differentially expressed between paired comparisons of libraries where 13,907 genes (48.12 %) were upregulated and 14,992 genes (51.88 %) were downregulated. DEGs between paired libraries were 106-5775 genes relative to the compared libraries (56-2982 and 50-2782 for upregulated and downregulated DEGs). Interestingly, 432 pigmentation genes of B. splendens were found. Of these, 297 DEGs showed differential expression between varieties. Many DEGs in melanogenesis (Bsmcr1r, Bsmcr5r, and Bsslc2a15b), tyrosine metabolism (Bstyr, Bstyrp1b and Bsdct), stripe repressor (BsAsip1 and BsAsip2b), pteridine (Bsgch2) and carotenoid (BsBco2) biosynthesis were downregulated in the Thai-flag compared with solid color varieties. Expression of Bsbco1l, Bsfrem2b, Bskcnj13, Bszic2a and Bspah in skin, muscle and tail of Thai-flag, Blue, Red and White varieties was analyzed by qRT-PCR and revealed differential expression between fish varieties and showed anatomical tissue-preferred expression patterns in the same fish variety. The information could be applied to assist genetic-based development of new B. splendens varieties in the future.
摘要：
在皮肤中表达的色素沉着基因，与蓝色相比，泰国国旗的身体肌肉和尾巴，确定了暹罗斗鱼斗鱼的白色和红色品种。总的来说,发现了22,919个新的单基因。Pearson相关和PCA分析显示，肌肉中基因的表达谱，整个纯色品种的皮肤和尾巴相似。相比之下,泰旗皮肤和红尾部分的表达密切相关，但与白尾部分的表达谱不同。此外，在鉴定的基因的外显子区域中鉴定了21,347-64,965个SNP。总的来说,28,899个基因在文库的配对比较之间差异表达，其中13,907个基因（48.12％）上调，14,992个基因（51.88％）下调。配对文库之间的DEG相对于比较文库是106-5775个基因(对于上调和下调的DEG,为56-2982和50-2782)。有趣的是,发现了432个Splendens的色素沉着基因。其中,297个DEGs在品种间表现出差异表达。黑色素生成中的许多DEGs(Bsmcr1r，Bsmcr5r,和Bsslc2a15b），酪氨酸代谢(Bstyr，Bstyrp1b和Bsdct)，条纹抑制因子（BsAsip1和BsAsip2b），与纯色品种相比，泰国国旗中的蝶啶（Bsgch2）和类胡萝卜素（BsBco2）的生物合成下调。Bsbco1l的表达，Bsfrem2b,Bskcnj13，Bszic2a和Bspah在皮肤，泰国国旗的肌肉和尾巴，蓝色,通过qRT-PCR分析红色和白色品种，揭示了鱼类品种之间的差异表达，并在同一鱼类品种中显示了解剖组织优选的表达模式。该信息可用于将来帮助新的B.splendens品种的基于遗传的开发。
