Abstract：
Hypertension remains a significant global health concern, contributing significantly to cardiovascular diseases and mortality rates. The inhibition of angiotensin-converting enzyme (ACE) plays a crucial role in alleviating high blood pressure. We investigated the potential of finger millets (Eleusine coracana) as a natural remedy for hypertension by isolating and characterizing its ACE-inhibitory compound. First, we evaluated the ACE-inhibitory activity of the finger millet ethanol extract and subsequently proceeded with solvent fractionation. Among the solvent fractions, the ethyl acetate fraction exhibited the highest ACE inhibitory activity and was further fractionated. Using preparative high-performance liquid chromatography, the ethyl acetate fraction was separated into four subfractions, with fraction 2 (F2) exhibiting the highest ACE inhibitory activity. Subsequent 1 H-nuclear magnetic resonance (NMR) and 13 C-NMR analyses confirmed that the isolated compound from F2 was catechin. Furthermore, molecular docking studies indicated that catechin has the potential to act as an ACE inhibitor. These findings suggest that finger millets, particularly as a source of catechin, have the potential to be used as a natural antihypertensive.
摘要：
高血压仍然是一个重要的全球健康问题，对心血管疾病和死亡率有显著影响。抑制血管紧张素转换酶(ACE)对缓解高血压具有重要作用。我们通过分离和表征其ACE抑制性化合物，研究了小米（Eleusinecoracana）作为高血压的自然疗法的潜力。首先,我们评估了小米乙醇提取物的ACE抑制活性，随后进行了溶剂分馏。在溶剂馏分中，乙酸乙酯部分表现出最高的ACE抑制活性，并进一步分馏。采用制备型高效液相色谱法,将乙酸乙酯部分分离成四个亚部分，其中级分2(F2)表现出最高的ACE抑制活性。随后的1H-核磁共振(NMR)和13C-NMR分析证实从F2分离的化合物是儿茶素。此外，分子对接研究表明儿茶素具有作为ACE抑制剂的潜力。这些发现表明，手指小米，特别是作为儿茶素的来源，有可能被用作天然的抗高血压药。
