Abstract：
Three compounds with fluorescence quantum yields in the range of 10- 5 to 10- 4 and emission spectra covering the UV/Vis spectral range are suggested as new references for the determination of small fluorescence quantum yields. The compounds are thymidine (dT) in water, dibenzoylmethane (DBM) in ethanol, and malachite green chloride (MG) in water, representing the blue, green, and red regions of the spectrum, respectively. All compounds are easily handled, photostable, and commercially available. Furthermore, these compounds exhibit a mirror-image symmetry between their absorption and fluorescence spectra. This symmetry, along with closely aligned fluorescence excitation and absorption spectra, confirms that the observed emissions originate from the compounds themselves. The fluorescence quantum yields were determined via a relative approach as well as Strickler-Berg analysis in conjunction with time resolved fluorescence spectroscopy. Within the respective error margins, the two approaches yielded identical results.
摘要：
提出了三种荧光量子产率在10-5至10-4范围内，发射光谱覆盖UV/Vis光谱范围的化合物，作为测定小荧光量子产率的新参考。该化合物是在水中的胸苷（dT），二苯甲酰甲烷（DBM）在乙醇中，和孔雀石绿氯化物（MG）在水中，代表蓝色，绿色,和光谱的红色区域，分别。所有化合物都很容易处理，光稳定，和商业可用。此外，这些化合物在其吸收和荧光光谱之间表现出镜像对称。这种对称性，以及紧密对齐的荧光激发和吸收光谱，证实了观察到的排放来自化合物本身。荧光量子产率通过相对方法以及Strickler-Berg分析结合时间分辨荧光光谱法确定。在各自的误差范围内，这两种方法产生了相同的结果。
