Abstract：
UNASSIGNED: To investigate corneal biomechanical changes after corneal cross-linking (CXL) treatments with rose bengal-green light (RB-CXL) and riboflavin-UVA (RF-CXL).
UNASSIGNED: A total of 60 freshly enucleated lamb eyes were obtained for this experimental study. Fifteen eyes were treated with RB-CXL using 0.1% RB solution (Group 1), 15 eyes were treated with RB-CXL using 0.2% RB solution (Group 2), 15 eyes were treated with RF-CXL using 0.1% RF solution (Group 3), and 15 eyes were used as controls (Group 4). The same treatment protocol (10-minute irradiation using a total of 5.4 J/cm2 energy) was applied to all treatment groups. To evaluate corneal biomechanical changes, the stress-strain test was used for both the treated and control corneas. The elastic modulus was calculated using the tension strain curves obtained during the test.
UNASSIGNED: The average elastic modulus values were calculated to be 18.9, 23.5, 22.3, and 14.1 MPa in Groups 1, 2, 3, and 4, respectively. Statistically significant differences were found between the groups (p < 0.001 for Group 1 vs. 2; p < 0.001 for Group 1 vs. 3; p < 0.001 for Group 1 vs. 4; p = 0.002 for Group 2 vs. 3; p < 0.001 for Group 2 vs. 4; and p < 0.001 for Group 3 vs. 4).
UNASSIGNED: In this study, the efficacy of RB-CXL treatment applied using different concentrations of RB solutions at a total energy of 5.4 J/cm2 was investigated, and 0.2% RB solution was found to have at least as much and even more effective than the RF-CXL (0.1% RF) on the corneal elasticity module. These results are encouraging for the treatment of ectatic corneas particularly below 400 μm. It is considered that the findings obtained from this study will guide future experimental and clinical studies.
UNASSIGNED: A total of 60 freshly enucleated lamb eyes were obtained for this experimental study. Fifteen eyes were treated with RB-CXL using 0.1% RB solution (Group 1), 15 eyes were treated with RB-CXL using 0.2% RB solution (Group 2), 15 eyes were treated with RF-CXL using 0.1% RF solution (Group 3), and 15 eyes were used as controls (Group 4). The same treatment protocol (10-minute irradiation using a total of 5.4 J/cm2 energy) was applied to all treatment groups. To evaluate corneal biomechanical changes, the stress-strain test was used for both the treated and control corneas. The elastic modulus was calculated using the tension strain curves obtained during the test.
UNASSIGNED: The average elastic modulus values were calculated to be 18.9, 23.5, 22.3, and 14.1 MPa in Groups 1, 2, 3, and 4, respectively. Statistically significant differences were found between the groups (p < 0.001 for Group 1 vs. 2; p < 0.001 for Group 1 vs. 3; p < 0.001 for Group 1 vs. 4; p = 0.002 for Group 2 vs. 3; p < 0.001 for Group 2 vs. 4; and p < 0.001 for Group 3 vs. 4).
UNASSIGNED: In this study, the efficacy of RB-CXL treatment applied using different concentrations of RB solutions at a total energy of 5.4 J/cm2 was investigated, and 0.2% RB solution was found to have at least as much and even more effective than the RF-CXL (0.1% RF) on the corneal elasticity module. These results are encouraging for the treatment of ectatic corneas particularly below 400 μm. It is considered that the findings obtained from this study will guide future experimental and clinical studies.
摘要：
■研究玫瑰红绿光（RB-CXL）和核黄素-UVA（RF-CXL）角膜交联（CXL）治疗后的角膜生物力学变化。
■本实验研究共获得60只新鲜摘除的羔羊眼。用0.1%RB溶液用RB-CXL治疗15只眼（第1组），15只眼使用0.2%RB溶液用RB-CXL治疗（第2组），15只眼用0.1%RF溶液用RF-CXL治疗(第3组),15只眼作为对照(第4组)。将相同的处理方案(使用总共5.4J/cm2能量的10分钟照射)应用于所有处理组。评估角膜生物力学变化，应力-应变试验用于治疗和对照角膜.使用在测试期间获得的拉伸应变曲线计算弹性模量。
■计算出第1、2、3和4组中的平均弹性模量值分别为18.9、23.5、22.3和14.1MPa。两组之间存在统计学上的显着差异（第1组的p<0.001与2；第1组与第1组的p<0.0013；第1组与第1组的p<0.0014；第2组与第2组的p=0.0023；第2组与第2组的p<0.0014；第3组与第3组的p<0.0014).
■在这项研究中，研究了使用不同浓度的RB溶液在总能量为5.4J/cm2时应用的RB-CXL处理的功效，发现0.2%RB溶液在角膜弹性模块上具有与RF-CXL(0.1%RF)至少一样多甚至更有效的效果。这些结果对于特别是低于400μm的外生性角膜的治疗是令人鼓舞的。认为从这项研究中获得的发现将指导未来的实验和临床研究。
■本实验研究共获得60只新鲜摘除的羔羊眼。用0.1%RB溶液用RB-CXL治疗15只眼（第1组），15只眼使用0.2%RB溶液用RB-CXL治疗（第2组），15只眼用0.1%RF溶液用RF-CXL治疗(第3组),15只眼作为对照(第4组)。将相同的处理方案(使用总共5.4J/cm2能量的10分钟照射)应用于所有处理组。评估角膜生物力学变化，应力-应变试验用于治疗和对照角膜.使用在测试期间获得的拉伸应变曲线计算弹性模量。
■计算出第1、2、3和4组中的平均弹性模量值分别为18.9、23.5、22.3和14.1MPa。两组之间存在统计学上的显着差异（第1组的p<0.001与2；第1组与第1组的p<0.0013；第1组与第1组的p<0.0014；第2组与第2组的p=0.0023；第2组与第2组的p<0.0014；第3组与第3组的p<0.0014).
■在这项研究中，研究了使用不同浓度的RB溶液在总能量为5.4J/cm2时应用的RB-CXL处理的功效，发现0.2%RB溶液在角膜弹性模块上具有与RF-CXL(0.1%RF)至少一样多甚至更有效的效果。这些结果对于特别是低于400μm的外生性角膜的治疗是令人鼓舞的。认为从这项研究中获得的发现将指导未来的实验和临床研究。
