Abstract：
Plant breeders are generally reluctant to cross elite crop cultivars with their wild relatives to introgress novel desirable traits due to associated negative traits such as pod shattering. This results in a genetic bottleneck that could be reduced through better understanding of the genomic locations of the gene(s) controlling this trait. We integrated information on parental genomes, pod shattering data from multiple environments, and high-density genetic linkage maps to identify pod shattering quantitative trait loci (QTLs) in three lentil interspecific recombinant inbred line populations. The broad-sense heritability on a multi-environment basis varied from 0.46 (in LR-70, Lens culinaris × Lens odemensis) to 0.77 (in LR-68, Lens orientalis × L. culinaris). Genetic linkage maps of the interspecific populations revealed reciprocal translocations of chromosomal segments that differed among the populations, and which were associated with reduced recombination. LR-68 had a 2-5 translocation, LR-70 had 1-5, 2-6, and 2-7 translocations, and LR-86 had a 2-7 translocation in one parent relative to the other. Segregation distortion was also observed for clusters of single nucleotide polymorphisms on multiple chromosomes per population, further affecting introgression. Two major QTL, on chromosomes 4 and 7, were repeatedly detected in the three populations and contain several candidate genes. These findings will be of significant value for lentil breeders to strategically access novel superior alleles while minimizing the genetic impact of pod shattering from wild parents.
摘要：
由于相关的负面特征，例如豆荚破碎，植物育种者通常不愿将优良作物品种与其野生近缘种杂交以引入新的理想特征。这导致可以通过更好地理解控制该性状的基因的基因组位置来减少的遗传瓶颈。我们整合了父母基因组的信息，Pod会破坏来自多个环境的数据，和高密度遗传连锁图谱，以鉴定三个小扁豆种间重组自交系种群中的豆荚破碎数量性状位点（QTLs）。在多环境基础上的广义遗传力从0.46（在LR-70中，透镜体×透镜体）到0.77（在LR-68中，透镜体×L体）不等。种间种群的遗传连锁图谱揭示了种群之间不同的染色体片段的相互易位，与重组减少有关。LR-68有2-5易位,LR-70有1-5、2-6和2-7个易位，和LR-86有2-7易位在一个父母相对于另一个。每个种群的多个染色体上的单核苷酸多态性簇也观察到了分离畸变，进一步影响基因渗入。两个主要的QTL，在4号和7号染色体上，在三个种群中反复检测到，并含有几个候选基因。这些发现对于小扁豆育种者战略性地获得新的优良等位基因，同时最大程度地减少野生亲本豆荚破碎的遗传影响具有重要价值。
