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Abstract：
This study investigated the synthesis of bioactive peptides from sheep milk through fermentation with Limosilactobacillus fermentum KGL4 MTCC 25515 strain and assessed lipase inhibition, ACE inhibition, α-glucosidase inhibition, and α-amylase inhibition activities during the fermentation process. The study observed the highest activities, reaching 74.82%, 70.02%, 72.19%, and 67.08% (lipase inhibition, ACE inhibition, α-glucosidase inhibition, and α-amylase inhibition) after 48 h at 37°C, respectively. Growth optimization experiments revealed that a 2.5% inoculation rate after 48 h of fermentation time resulted in the highest proteolytic activity at 9.88 mg/mL. Additionally, fractions with less than 3 kDa of molecular weight exhibited superior ACE-inhibition and anti-diabetic activities compared to other fractions. Fermentation of sheep milk with KGL4 led to a significant reduction in the excessive production of NO, TNF-α, IL-6, and IL-1β produced in RAW 267.4 cells upon treatment with LPS. Peptides were purified utilizing SDS-PAGE and electrophoresis on 2D gels, identifying a maximum number of proteins bands ranging 10-70 kDa. Peptide sequences were cross-referenced with AHTPDB and BIOPEP databases, confirming potential antihypertensive and antidiabetic properties. Notably, the peptide (GPFPILV) exhibited the highest HPEPDOCK score against both α-amylase and ACE.
摘要：
本研究研究了通过发酵LimosilactacillusKGL4MTCC25515菌株发酵从羊乳中合成生物活性肽，并评估了脂肪酶抑制作用。ACE抑制,α-葡萄糖苷酶抑制，发酵过程中的α-淀粉酶抑制活性。这项研究观察到了最高的活动，达到74.82%，70.02%,72.19%，和67.08%(脂肪酶抑制，ACE抑制,α-葡萄糖苷酶抑制，和α-淀粉酶抑制)在37°C下48小时后，分别。生长优化实验表明，发酵48小时后的2.5％接种率导致最高的蛋白水解活性为9.88mg/mL。此外,与其他部分相比，分子量小于3kDa的部分表现出优异的ACE抑制和抗糖尿病活性。用KGL4发酵羊奶导致NO的过量产生显著减少，TNF-α,IL-6和IL-1β在用LPS处理后在RAW267.4细胞中产生。利用SDS-PAGE和2D凝胶电泳纯化肽，鉴定范围为10-70kDa的蛋白质条带的最大数目。肽序列与AHTPDB和BIOPEP数据库交叉引用，确认潜在的抗高血压和抗糖尿病特性。值得注意的是,肽（GPFPILV）对α-淀粉酶和ACE均表现出最高的HPEPDOCK评分。
