PDF(Pubmed)
Abstract：
This study manufactured a 35 kDa hyaluronan fragment (HA35) by enzymatically degrading high-molecular-weight HA using hyaluronidase PH20 derived from bovine testis. The research then examined the therapeutic efficacy of locally administered, tissue-permeable HA35 in alleviating chronic wounds and their associated neuropathic pain. For 20 patients with nonhealing wounds and associated pain lasting over three months, 100 mg of HA35 was injected daily into the healthy skin surrounding the chronic wound for 10 days. Self-assessments before and after treatment indicated that HA35 significantly enhanced wound healing. This was evidenced by the formation of fresh granulation tissue on the wounds (p < 0.0001); reduced darkness, redness, dryness, and damage in the skin surrounding the wounds (p < 0.0001), and a decrease in wound size (p < 0.001). Remarkably, HA35 injections alleviated pain associated with chronic wounds within 24 hours (p < 0.0001). It can be concluded that the low-molecular-weight hyaluronan fragment HA35 potentially enhances the immune response and angiogenesis during wound healing.
摘要：
这项研究通过使用来自牛睾丸的透明质酸酶PH20酶促降解高分子量HA来制造35kDa透明质酸片段（HA35）。然后研究检查了局部给药的治疗效果，组织渗透性HA35减轻慢性伤口及其相关的神经性疼痛。对于20例伤口不愈合和相关疼痛持续三个月以上的患者，每天将100mgHA35注射到慢性伤口周围的健康皮肤中,持续10天。治疗前后的自我评估表明HA35显着增强了伤口愈合。伤口上形成新鲜的肉芽组织证明了这一点（p<0.0001）；减少了黑暗，发红,干燥度,以及伤口周围皮肤的损伤（p<0.0001），和伤口大小减小(p<0.001)。值得注意的是,HA35注射在24小时内减轻了与慢性伤口相关的疼痛(p<0.0001)。可以得出结论,低分子量透明质酸片段HA35潜在地增强伤口愈合期间的免疫应答和血管生成。
