Abstract：
BACKGROUND: Lung cancer is one of the most prevalent malignancies worldwide. Substantial research has illuminated the intricate interplay between microorganisms and human health, revealing their role in disease regulation. Trichomonads is a flagellated protozoan in the human cavity and have been previously identified as a pathogen associated with pneumonia, contributing to tissue chronic inflammation and carcinogenesis.
METHODS: Nested polymerase chain reaction methods were employed to scrutinize the prevalence of trichomonads in the bronchovesicular fluid of patients diagnosed with lung cancer. Subsequently, the influence of Trichomonas tenax invasion on lung cancer cells was elucidated through proliferation assays, migration assays, and transcription analysis.
RESULTS: Bronchoalveolar fluid samples from lung cancer patients yielded positive nested PCR results for eight out of twenty-seven samples. Seven of these samples were identified as Trichomonas tenax, while one was identified as Tetratrichomonas spp. Our findings revealed a significant upregulation of pathways associated with carcinogenesis, including cellular proliferation, migration, and drug resistance, in response to T. tenax invasion.
CONCLUSIONS: This study underscores the importance of recognizing the presence of trichomonads and the influence of T. tenax invasion on host responses to respiratory diseases. The identified pathways implicated in cancer development may pave the way for developing targeted treatment strategies for pulmonary diseases. These findings hold promise for informing and improving the precision of therapeutic interventions in the context of pulmonary ailments.
METHODS: Nested polymerase chain reaction methods were employed to scrutinize the prevalence of trichomonads in the bronchovesicular fluid of patients diagnosed with lung cancer. Subsequently, the influence of Trichomonas tenax invasion on lung cancer cells was elucidated through proliferation assays, migration assays, and transcription analysis.
RESULTS: Bronchoalveolar fluid samples from lung cancer patients yielded positive nested PCR results for eight out of twenty-seven samples. Seven of these samples were identified as Trichomonas tenax, while one was identified as Tetratrichomonas spp. Our findings revealed a significant upregulation of pathways associated with carcinogenesis, including cellular proliferation, migration, and drug resistance, in response to T. tenax invasion.
CONCLUSIONS: This study underscores the importance of recognizing the presence of trichomonads and the influence of T. tenax invasion on host responses to respiratory diseases. The identified pathways implicated in cancer development may pave the way for developing targeted treatment strategies for pulmonary diseases. These findings hold promise for informing and improving the precision of therapeutic interventions in the context of pulmonary ailments.
摘要：
背景：肺癌是全球最常见的恶性肿瘤之一。大量的研究阐明了微生物与人类健康之间错综复杂的相互作用，揭示它们在疾病调节中的作用。毛滴虫是人腔中的鞭毛原生动物，先前已被确定为与肺炎相关的病原体，有助于组织慢性炎症和癌变。
方法：采用巢式聚合酶链反应方法来检查诊断为肺癌的患者支气管囊液中滴虫的患病率。随后，通过增殖试验阐明了毛滴虫侵袭对肺癌细胞的影响，迁移测定，和转录分析。
结果：来自肺癌患者的支气管肺泡液样本在27个样本中有8个产生了阳性巢式PCR结果。这些样本中有七个被鉴定为毛滴虫，而其中一个被鉴定为四虫属。我们的发现揭示了与癌变相关的途径的显着上调，包括细胞增殖，迁移,和抗药性，作为对T.Tenax入侵的回应。
结论：这项研究强调了认识滴虫的存在和T.tenax侵袭对宿主对呼吸道疾病反应的影响的重要性。确定的与癌症发展有关的途径可能为开发针对肺部疾病的靶向治疗策略铺平道路。这些发现有望在肺部疾病的背景下提供信息并提高治疗干预措施的准确性。
方法：采用巢式聚合酶链反应方法来检查诊断为肺癌的患者支气管囊液中滴虫的患病率。随后，通过增殖试验阐明了毛滴虫侵袭对肺癌细胞的影响，迁移测定，和转录分析。
结果：来自肺癌患者的支气管肺泡液样本在27个样本中有8个产生了阳性巢式PCR结果。这些样本中有七个被鉴定为毛滴虫，而其中一个被鉴定为四虫属。我们的发现揭示了与癌变相关的途径的显着上调，包括细胞增殖，迁移,和抗药性，作为对T.Tenax入侵的回应。
结论：这项研究强调了认识滴虫的存在和T.tenax侵袭对宿主对呼吸道疾病反应的影响的重要性。确定的与癌症发展有关的途径可能为开发针对肺部疾病的靶向治疗策略铺平道路。这些发现有望在肺部疾病的背景下提供信息并提高治疗干预措施的准确性。
