Abstract：
OBJECTIVE: To determine antibiotic levels in plasma and interstitial fluid (ISF) after SC placement of compounded florfenicol (FF) calcium sulfate beads (CSBs) in New Zealand White rabbits (Oryctolagus cuniculus).
METHODS: 6 juvenile female rabbits (n = 5 treatment and 1 control).
METHODS: An ultrafiltration probe and CSBs were placed SC in 6 rabbits (n = 5 for FF CSBs and 1 for control CSBs). Plasma (3, 6, 12, 24, and 48 hours and 7, 14, and 21 days) and ISF (daily for 21 days) samples were collected, and FF was measured by HPLC for pharmacokinetic analysis. Hematology, biochemistry, and histopathology were assessed.
RESULTS: Means ± SD for the area under the curve, maximum concentration, time of maximum concentration, terminal half-life, and mean residence time to the last data point for plasma and ISF were 16.63 ± 8.16 and 17,902 ± 7,564 h·µg/mL, 0.79 ± 0.38 and 245 ± 223 µg/mL, 2.90 ± 0.3 and 59 ± 40 hours, 30.81 ± 16.9 and 27.3 ± 9.39 hours, 23.4 ± 10 and 73.7 ± 13 hours, respectively. Plasma FF was < 2 µg/mL at all time points. The ISF FF remained > 8 μg/mL for 109.98 to 231.58 hours. One rabbit death occurred during treatment, but the cause of death was undetermined. Local tissue inflammation was present, but no clinically significant systemic adverse effects were found on hematology, biochemistry, or histopathology in the remaining rabbits.
CONCLUSIONS: Florfenicol CSBs maintained antibiotic concentrations in ISF at levels likely to be effective against bacteria sensitive to > 8 µg/mL for 5 to 10 days while maintaining low (< 2 µg/mL) plasma levels. Florfenicol CSBs may be effective for local antibiotic treatment in rabbit abscesses.
METHODS: 6 juvenile female rabbits (n = 5 treatment and 1 control).
METHODS: An ultrafiltration probe and CSBs were placed SC in 6 rabbits (n = 5 for FF CSBs and 1 for control CSBs). Plasma (3, 6, 12, 24, and 48 hours and 7, 14, and 21 days) and ISF (daily for 21 days) samples were collected, and FF was measured by HPLC for pharmacokinetic analysis. Hematology, biochemistry, and histopathology were assessed.
RESULTS: Means ± SD for the area under the curve, maximum concentration, time of maximum concentration, terminal half-life, and mean residence time to the last data point for plasma and ISF were 16.63 ± 8.16 and 17,902 ± 7,564 h·µg/mL, 0.79 ± 0.38 and 245 ± 223 µg/mL, 2.90 ± 0.3 and 59 ± 40 hours, 30.81 ± 16.9 and 27.3 ± 9.39 hours, 23.4 ± 10 and 73.7 ± 13 hours, respectively. Plasma FF was < 2 µg/mL at all time points. The ISF FF remained > 8 μg/mL for 109.98 to 231.58 hours. One rabbit death occurred during treatment, but the cause of death was undetermined. Local tissue inflammation was present, but no clinically significant systemic adverse effects were found on hematology, biochemistry, or histopathology in the remaining rabbits.
CONCLUSIONS: Florfenicol CSBs maintained antibiotic concentrations in ISF at levels likely to be effective against bacteria sensitive to > 8 µg/mL for 5 to 10 days while maintaining low (< 2 µg/mL) plasma levels. Florfenicol CSBs may be effective for local antibiotic treatment in rabbit abscesses.
摘要：
目的：测定复合氟苯尼考（FF）硫酸钙珠（CSB）在新西兰白兔（Oryctolaguscuniculus）中SC放置后血浆和间质液（ISF）中的抗生素水平。
方法:6只幼年雌性兔(n=5处理和1只对照)。
方法：将超滤探针和CSBSC放置在6只兔子中（对于FFCSB，n=5，对于对照CSB，n=1）。收集血浆（3、6、12、24和48小时以及7、14和21天）和ISF（每天21天）样品，和FF通过HPLC测量用于药代动力学分析。血液学,生物化学,并对组织病理学进行了评估。
结果：曲线下面积的平均值±SD，最大浓度,最大浓度时间，终末半衰期，血浆和ISF到最后一个数据点的平均停留时间为16.63±8.16和17,902±7,564小时·µg/mL，0.79±0.38和245±223µg/mL，2.90±0.3和59±40小时，30.81±16.9和27.3±9.39小时，23.4±10和73.7±13小时，分别。在所有时间点血浆FF<2μg/mL。ISFFF保持>8μg/mL持续109.98至231.58小时。一只兔子在治疗期间死亡，但死因尚未确定.局部组织出现炎症，但是在血液学上没有发现临床上明显的全身不良反应，生物化学,或其余兔子的组织病理学。
结论：氟苯尼考CSB将ISF中的抗生素浓度维持在可能对>8µg/mL敏感的细菌有效5至10天的水平，同时维持较低的（<2µg/mL）血浆水平。氟苯尼考CSBs可能对兔脓肿的局部抗生素治疗有效。
方法:6只幼年雌性兔(n=5处理和1只对照)。
方法：将超滤探针和CSBSC放置在6只兔子中（对于FFCSB，n=5，对于对照CSB，n=1）。收集血浆（3、6、12、24和48小时以及7、14和21天）和ISF（每天21天）样品，和FF通过HPLC测量用于药代动力学分析。血液学,生物化学,并对组织病理学进行了评估。
结果：曲线下面积的平均值±SD，最大浓度,最大浓度时间，终末半衰期，血浆和ISF到最后一个数据点的平均停留时间为16.63±8.16和17,902±7,564小时·µg/mL，0.79±0.38和245±223µg/mL，2.90±0.3和59±40小时，30.81±16.9和27.3±9.39小时，23.4±10和73.7±13小时，分别。在所有时间点血浆FF<2μg/mL。ISFFF保持>8μg/mL持续109.98至231.58小时。一只兔子在治疗期间死亡，但死因尚未确定.局部组织出现炎症，但是在血液学上没有发现临床上明显的全身不良反应，生物化学,或其余兔子的组织病理学。
结论：氟苯尼考CSB将ISF中的抗生素浓度维持在可能对>8µg/mL敏感的细菌有效5至10天的水平，同时维持较低的（<2µg/mL）血浆水平。氟苯尼考CSBs可能对兔脓肿的局部抗生素治疗有效。
